PUBLICATION
An active heteropolysaccharide from the rinds of Garcinia mangostana Linn.: Structural characterization and immunomodulation activity evaluation
- Authors
- Zhang, S., An, L., Li, Z., Wang, H., Shi, L., Zhang, J., Li, Y., Jin, D.Q., Tuerhong, M., Ohizumi, Y., Shuai, L., Xu, J., Guo, Y.
- ID
- ZDB-PUB-200304-29
- Date
- 2020
- Source
- Carbohydrate Polymers 235: 115929 (Journal)
- Registered Authors
- Li, Yuhao
- Keywords
- 2', 7'-dichlorofluorescein diacetate (PubChem CID: 104913), 3-amino, 4-aminomethyl-2′, 7′-difluorescein diacetate (PubChem CID: 2762646), Acetic anhydride (PubChem CID: 7918), Congo red (PubChem CID: 11313), Garcinia mangostana Linn., Immunomodulation, L-arabinose (PubChem CID: 439195), Macrophages, Methyl iodide (PubChem CID: 6328), Neutral red (PubChem CID: 11105), Polysaccharide, Potassium bromide (PubChem CID: 253877), Sodium borodeuteride (PubChem CID: 23673181), Trifluoroacetic acid (PubChem CID: 6422), Zebrafish embryo
- MeSH Terms
-
- Animals
- Carbohydrate Conformation
- Garcinia mangostana/chemistry*
- Immunomodulation
- Macrophages/immunology
- Mice
- Models, Immunological
- Nitric Oxide/immunology
- Particle Size
- Polysaccharides/chemistry
- Polysaccharides/immunology*
- Polysaccharides/isolation & purification
- RAW 264.7 Cells
- Reactive Oxygen Species/immunology
- Surface Properties
- Zebrafish
- PubMed
- 32122475 Full text @ Carbohydr. Polym.
Citation
Zhang, S., An, L., Li, Z., Wang, H., Shi, L., Zhang, J., Li, Y., Jin, D.Q., Tuerhong, M., Ohizumi, Y., Shuai, L., Xu, J., Guo, Y. (2020) An active heteropolysaccharide from the rinds of Garcinia mangostana Linn.: Structural characterization and immunomodulation activity evaluation. Carbohydrate Polymers. 235:115929.
Abstract
A previously undescribed polysaccharide, GMP70-1, was isolated from the rinds of Garcinia mangostana Lin. Physicochemical characterization analysis showed that GMP70-1 (absolute molecular weight: 2.01 × 104 g/mol) is a multi-branched acidic heteropolysaccharide with a compact coil chain conformation in sodium chloride solution. The repeated unit of GMP70-1 was mainly composed of (1 → 5)-linked α-L-Araf, (1 → 3, 5)-linked α-L-Araf, (1 → 2, 4)-linked α-L-Rhap, (1 → 4)-linked β-D-Galp, terminating with t-α-L-Araf, t-α-D-GalpA, and t-β-D-Galp. To explore the medicinal potential responsible for the bioactivity of G. mangostana, an immunomodulatory assay was performed. The in vitro cell test showed that GMP70-1 possessed a prominent immunoregulatory activity by enhancing the phagocytic uptake of neutral red and promoting the secretion of nitric oxide (NO), reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β of macrophages. Furthermore, an in vivo zebrafish evaluation revealed that the production of ROS and NO was significantly increased after treated with GMP70-1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping