PUBLICATION

Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis

Authors
Sato, Y., Hilbert, L., Oda, H., Wan, Y., Heddleston, J.M., Chew, T.L., Zaburdaev, V., Keller, P., Lionnet, T., Vastenhouw, N., Kimura, H.
ID
ZDB-PUB-191002-2
Date
2019
Source
Development (Cambridge, England)   146(19): (Journal)
Registered Authors
Keller, Philipp, Vastenhouw, Nadine, Wan, Yinan
Keywords
Chromatin regulation, Histone modifications, Live-cell imaging, Zygotic genome activation
MeSH Terms
  • Acetylation/drug effects
  • Alpha-Amanitin/pharmacology
  • Animals
  • Cell Nucleus/drug effects
  • Cell Nucleus/metabolism
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental*/drug effects
  • Genome*
  • Histone Code/drug effects
  • Histones/metabolism*
  • Lysine/metabolism*
  • RNA Polymerase II/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Transcription, Genetic*/drug effects
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zygote/drug effects
  • Zygote/metabolism*
PubMed
31570370 Full text @ Development
Abstract
Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA) in living zebrafish embryos. Among various histone modifications, H3 Lys27 acetylation (H3K27ac) exhibited most drastic changes, accumulating in two nuclear foci in the 64- to 1k-cell-stage embryos. The elongating form of RNA polymerase II, which is phosphorylated at Ser2 in heptad repeats within the C-terminal domain (RNAP2 Ser2ph), and miR-430 transcripts were also concentrated in foci closely associated with H3K27ac. When treated with α-amanitin to inhibit transcription or JQ-1 to inhibit binding of acetyl-reader proteins, H3K27ac foci still appeared but RNAP2 Ser2ph and miR-430 morpholino were not concentrated in foci, suggesting that H3K27ac precedes active transcription during ZGA. We anticipate that the method presented here could be applied to a variety of developmental processes in any model and non-model organisms.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping