PUBLICATION

Triclosan affects Ca2+ regulatory module and musculature development in skeletal myocyte during early life stage of zebrafish (Danio rerio)

Authors
Ma, Z., Liu, H., Yu, H.
ID
ZDB-PUB-190919-14
Date
2019
Source
Environmental science & technology   53(20): 11988-11998 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Animals
  • Calcium Channels, L-Type
  • Muscle Fibers, Skeletal
  • Muscle, Skeletal
  • Ryanodine Receptor Calcium Release Channel
  • Triclosan*
  • Zebrafish
PubMed
31532625 Full text @ Env. Sci. Tech.
Abstract
Advanced technologies for toxicity test are designed to identify biomarkers with superior predictive power or endpoints of the complex web of biological pathways. However, the data obtained need to be fully characterized for dose-response, physiological systems, and relevance to a system or (sub) population before biological interpretation and decision making. In this study, the toxicity of triclosan (TCS) on zebrafish was selected as a case study to correlate the observed morphological effects with existing data and identify the critical events by receptor activity sensitivity analysis. Triclosan exhibited weak acute toxicity against zebrafish and significantly affected the development of trunk muscles at 0.52, 1.04, and 1.73 μM. Through receptor-mediated screening, we found that the adverse effects of TCS induce Ryanodine receptor 1 (RyR1) activity and distort Ca2+ signaling. The trunk skeletal muscle abnormalities occurred only when dihydropyridine receptor (DHPR) was blocked, demonstrating that TCS mainly influenced Ca2+ regulatory module associated with signaling between DHPRs and RyR1; DHPRs mainly regulated the orthograde and retrograde signaling in skeletal muscles. This unexpected result could integrate the mode of action of TCS and provide insight for high-throughput screening and toxicity prediction using zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes