PUBLICATION
microRNA-29b knocks down collagen type I production in cultured rainbow trout (Oncorhynchus mykiss) cardiac fibroblasts
- Authors
- Johnston, E.F., Cadonic, I.G., Craig, P.M., Gillis, T.E.
- ID
- ZDB-PUB-190826-2
- Date
- 2019
- Source
- The Journal of experimental biology 222(Pt 17): (Journal)
- Registered Authors
- Keywords
- Cardiac remodeling, Collagen, Cultured fibroblasts, Extracellular matrix, Transcriptional regulation, microRNA
- MeSH Terms
-
- Animals
- Cells, Cultured
- Collagen Type I/metabolism*
- Fibroblasts/metabolism
- MicroRNAs/metabolism*
- Myocardium/metabolism*
- Oncorhynchus mykiss/metabolism*
- PubMed
- 31439649 Full text @ J. Exp. Biol.
Citation
Johnston, E.F., Cadonic, I.G., Craig, P.M., Gillis, T.E. (2019) microRNA-29b knocks down collagen type I production in cultured rainbow trout (Oncorhynchus mykiss) cardiac fibroblasts. The Journal of experimental biology. 222(Pt 17):.
Abstract
Warm acclimation of rainbow trout can cause a decrease in the collagen content of the heart. This ability to remove cardiac collagen is particularly interesting considering that collagen deposition in the mammalian heart, following an injury, is permanent. We hypothesized that collagen removal can be facilitated by microRNA-29b, a highly conserved, small, non-coding RNA, as a reduction in this microRNA has been reported during the development of fibrosis in the mammalian heart. We also used a bioinformatics approach to investigate the binding potential of miR-29b to the seed sequences of vertebrate collagen isoforms. Cultured trout cardiac fibroblasts were transfected with zebrafish mature miR-29b mimic for 7 d with re-transfection occurring after 3 d. Transfection induced a 17.8-fold increase in miR-29b transcript abundance (P<0.05) as well as a 54% decrease in the transcript levels of col1a3, compared to non-transfected controls (P<0.05). Western blotting demonstrated that the levels of collagen type I protein was 85% lower in cells transfected with miR-29b than in control cells (P<0.05). Finally, bioinformatic analysis suggested that the predicted 3'UTR of rainbow trout col1a3 has a comparatively higher binding affinity for miR-29b than the 3'UTR of col1a1 Together these results suggest that miR-29b is a highly conserved regulator of collagen type I protein in vertebrates and that this microRNA decreases collagen in the trout heart by targeting col1a3.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping