PUBLICATION

Cell and tissue morphology determine actin-dependent nuclear migration mechanisms in neuroepithelia

Authors
Yanakieva, I., Erzberger, A., Matejčić, M., Modes, C.D., Norden, C.
ID
ZDB-PUB-190820-1
Date
2019
Source
The Journal of cell biology   218(10): 3272-3289 (Journal)
Registered Authors
Erzberger, Anna, Norden, Caren, Yanakieva, Iskra
Keywords
none
MeSH Terms
  • Actins/metabolism*
  • Animals
  • Cell Movement*
  • Cell Nucleus/metabolism*
  • Neuroepithelial Cells/cytology*
  • Neuroepithelial Cells/metabolism*
  • Rats
  • Zebrafish
PubMed
31420451 Full text @ J. Cell Biol.
Abstract
Correct nuclear position is crucial for cellular function and tissue development. Depending on cell context, however, the cytoskeletal elements responsible for nuclear positioning vary. While these cytoskeletal mechanisms have been intensely studied in single cells, how nuclear positioning is linked to tissue morphology is less clear. Here, we compare apical nuclear positioning in zebrafish neuroepithelia. We find that kinetics and actin-dependent mechanisms of nuclear positioning vary in tissues of different morphology. In straight neuroepithelia, nuclear positioning is controlled by Rho-ROCK-dependent myosin contractility. In contrast, in basally constricted neuroepithelia, a novel formin-dependent pushing mechanism is found for which we propose a proof-of-principle force generation theory. Overall, our data suggest that correct nuclear positioning is ensured by the adaptability of the cytoskeleton to cell and tissue shape. This in turn leads to robust epithelial maturation across geometries. The conclusion that different nuclear positioning mechanisms are favored in tissues of different morphology highlights the importance of developmental context for the execution of intracellular processes.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping