PUBLICATION

Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish

Authors
Sanz-Morejón, A., García-Redondo, A.B., Reuter, H., Marques, I.J., Bates, T., Galardi-Castilla, M., Große, A., Manig, S., Langa, X., Ernst, A., Piragyte, I., Botos, M.A., González-Rosa, J.M., Ruiz-Ortega, M., Briones, A.M., Salaices, M., Englert, C., Mercader, N.
ID
ZDB-PUB-190801-25
Date
2019
Source
Cell Reports   28: 1296-1306.e6 (Journal)
Registered Authors
Englert, Christoph, Gonzalez-Rosa, Juan Manuel, Marques, Ines, Mercader Huber, Nadia
Keywords
Wilms tumor 1b, fin regeneration, heart regeneration, macrophages, zebrafish
Datasets
GEO:GSE118457, GEO:GSE118509, GEO:GSE115381
MeSH Terms
  • Animal Fins/physiology*
  • Animals
  • Heart/physiology*
  • Macrophages/cytology
  • Macrophages/metabolism*
  • Regeneration*
  • WT1 Proteins/genetics
  • WT1 Proteins/metabolism*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
31365871 Full text @ Cell Rep.
Abstract
Organ regeneration is preceded by the recruitment of innate immune cells, which play an active role during repair and regrowth. Here, we studied macrophage subtypes during organ regeneration in the zebrafish, an animal model with a high regenerative capacity. We identified a macrophage subpopulation expressing Wilms tumor 1b (wt1b), which accumulates within regenerating tissues. This wt1b+ macrophage population exhibited an overall pro-regenerative gene expression profile and different migratory behavior compared to the remainder of the macrophages. Functional studies showed that wt1b regulates macrophage migration and retention at the injury area. Furthermore, wt1b-null mutant zebrafish presented signs of impaired macrophage differentiation, delayed fin growth upon caudal fin amputation, and reduced cardiomyocyte proliferation following cardiac injury that correlated with altered macrophage recruitment to the regenerating areas. We describe a pro-regenerative macrophage subtype in the zebrafish and a role for wt1b in organ regeneration.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes