PUBLICATION
Intra- and interspecies comparison of EYS transcripts highlights its characteristics in the eye
- Authors
- Takita, S., Miyamoto-Matsui, K., Seko, Y.
- ID
- ZDB-PUB-190524-9
- Date
- 2019
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33(8): 9422-9433 (Journal)
- Registered Authors
- Miyamoto-Matsui, Kiyoko, Seko, Yuko, Takita, Shimpei
- Keywords
- HDF-a, Y79, domain structure, photoreceptors, zebrafish eye
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Cell Line, Tumor
- Cells, Cultured
- Computational Biology
- DNA Mutational Analysis
- Eye/metabolism*
- Eye Proteins/genetics
- Eye Proteins/metabolism*
- Fibroblasts/metabolism
- Humans
- Sequence Analysis, DNA
- Zebrafish
- PubMed
- 31120796 Full text @ FASEB J.
Citation
Takita, S., Miyamoto-Matsui, K., Seko, Y. (2019) Intra- and interspecies comparison of EYS transcripts highlights its characteristics in the eye. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 33(8):9422-9433.
Abstract
Inherited mutations in the eyes shut homolog (EYS) gene cause retinitis pigmentosa. Although knock out of eys in zebrafish is pathogenic, the molecular function of EYS in vertebrate photoreceptors is poorly understood. Here, we show that the 5' portion of EYS is eye-specific across vertebrates. We previously determined that a 3' fragment of EYS with an unknown transcription start site is expressed in human dermal fibroblasts (HDF). To obtain insights into the molecular function of EYS in vertebrate photoreceptors, we extensively analyzed EYS (eys) expression in the human fibroblast cell line HDF-adult (HDF-a), the Y79 retinoblastoma cell line, and in zebrafish eyes using rapid amplification of cDNA end, cap analysis of gene expression, RNA sequencing, and RT-PCR. In HDF-a cells, we identified a novel transcript variant (tv), tv5, transcribed from exon 37. In Y79 cells and zebrafish eyes, EYS (eys) was predominantly transcribed from exon 1 or 2, whereas it was transcribed exclusively from exon 37 in HDF-a cells. In the zebrafish eye, there were splice variants that introduced stop codons, resulting in complete loss of the 3' portion of the RNA. These comparative approaches indicate that the 5' portion of the EYS (eys) mRNA appears to be photoreceptor-specific and that the compositions of the deduced EYS proteins in the eye are well-conserved across vertebrates.-Takita, S., Miyamoto-Matsui, K., Seko, Y. Intra- and interspecies comparison of EYS transcripts highlights its characteristics in the eye.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping