PUBLICATION
Neurotensin Enhances Locomotor Activity and Arousal, and Inhibits Melanin-Concentrating Hormone Signalings
- Authors
- Levitas-Djerbi, T., Sagi, D., Lebenthal-Loinger, I., Lerer-Goldshtein, T., Appelbaum, L.
- ID
- ZDB-PUB-190429-8
- Date
- 2019
- Source
- Neuroendocrinology 110(1-2): 35-49 (Journal)
- Registered Authors
- Appelbaum, Lior, Levitas-Djerbi, Tali, Sagi, Dana
- Keywords
- none
- MeSH Terms
-
- Animals
- Arousal/physiology*
- Hypothalamic Hormones/metabolism*
- Locomotion/physiology*
- Melanins/metabolism*
- Neurotensin/metabolism
- Neurotensin/physiology*
- Pituitary Hormones/metabolism*
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- PubMed
- 31030196 Full text @ Neuroendocrinology
Citation
Levitas-Djerbi, T., Sagi, D., Lebenthal-Loinger, I., Lerer-Goldshtein, T., Appelbaum, L. (2019) Neurotensin Enhances Locomotor Activity and Arousal, and Inhibits Melanin-Concentrating Hormone Signalings. Neuroendocrinology. 110(1-2):35-49.
Abstract
Background: Hypothalamic neurotensin (Nts)-secreting neurons regulate fundamental physiological processes including metabolism and feeding. However, the role of Nts in modulation of locomotor activity, sleep and arousal is unclear. We previously identified and characterized Nts neurons in the zebrafish hypothalamus.
Materials and methods In order to study the role of Nts, nts mutant (nts-/-) and overexpressing zebrafish were generated.
Results The expression of both nts mRNA and Nts protein were reduced during the night in wild type zebrafish. Behavioral assays revealed that locomotor activity was decreased during both day and night, while sleep was increased exclusively during the night-time in nts-/- larvae. Likewise, inducible overexpression of Nts increased arousal in hsp70:Gal4/uas:Nts larvae. Furthermore, the behavioral response to light-to-dark transitions was reduced in nts-/- larvae. In order to elucidate potential contenders that may mediate Nts action on these behaviors, we profiled the transcriptome of 6 dpf nts-/- larvae. Among other genes, the expression levels of melanin-concentrating hormone receptor 1b (mchr1b) were increased in nts-/- larvae. Furthermore, a portion of pmch1 and pmch2 neurons express the nts receptor (ntsr). In addition, the expression of the two zebrafish Mch orthologs, Mch1 and Mch2, was increased in nts-/- larvae.
Conclusion These results show that that the Nts and Mch systems interact and modulate locomotor activity and arousal.<br>.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping