PUBLICATION
Anatomical assessment of the adult skeleton of zebrafish reared under different thyroid hormone profiles
- Authors
- Keer, S., Cohen, K., May, C., Hu, Y., McMenamin, S., Hernandez, L.P.
- ID
- ZDB-PUB-190418-7
- Date
- 2019
- Source
- Anatomical record (Hoboken, N.J. : 2007) 302(10): 1754-1769 (Journal)
- Registered Authors
- McMenamin, Sarah
- Keywords
- thyroid hormone, skeleton, Danio rerio, Weberian apparatus, craniofacial
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Models, Animal
- Osteogenesis/physiology*
- Skeleton/growth & development*
- Thyroid Hormones/genetics
- Thyroid Hormones/metabolism*
- Zebrafish/growth & development*
- PubMed
- 30989809 Full text @ Anat. Rec. (Hoboken)
Citation
Keer, S., Cohen, K., May, C., Hu, Y., McMenamin, S., Hernandez, L.P. (2019) Anatomical assessment of the adult skeleton of zebrafish reared under different thyroid hormone profiles. Anatomical record (Hoboken, N.J. : 2007). 302(10):1754-1769.
Abstract
Thyroid hormone (TH) directs the growth and maintenance of tissues throughout the body during development and into adulthood, and plays a particularly important role in proper ossification and homeostasis of the skeleton. To better understand the roles of TH in the skeletogenesis of a vertebrate model, and to define areas of the skeleton that are particularly sensitive to developmental TH, we examined the effects of hypo- and hyperthyroidism on skeletal development in zebrafish. Performing a bone-by-bone anatomical assessment on the entire skeleton of adult fish, we found that TH is required for proper ossification, growth, morphogenesis, and fusion of numerous bones. We showed that the pectoral girdle, dermatocranium, Weberian apparatus, and dentary are particularly sensitive to TH, and that TH affects development of skeletal element regardless of bone type and developmental origin. Indeed, the hormone does not universally promote ossification: we found that developmental TH prevents ectopic ossification in multiple thin bones and within connective tissue of the jaw. In all, we found that TH regulates proper morphogenesis and ossification in the majority of zebrafish bones, and that the requirement for the hormone extends across bone types and developmental profiles. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping