PUBLICATION
Scale invariance of BMP signaling gradients in zebrafish
- Authors
- Huang, Y., Umulis, D.M.
- ID
- ZDB-PUB-190402-14
- Date
- 2019
- Source
- Scientific Reports 9: 5440 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Body Patterning
- Bone Morphogenetic Proteins/metabolism*
- Embryo, Nonmammalian/metabolism
- Morphogenesis
- Signal Transduction
- Zebrafish/embryology
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism*
- PubMed
- 30932076 Full text @ Sci. Rep.
Citation
Huang, Y., Umulis, D.M. (2019) Scale invariance of BMP signaling gradients in zebrafish. Scientific Reports. 9:5440.
Abstract
In both vertebrates and invertebrates, spatial patterning along the Dorsal-ventral (DV) embryonic axis depends on a morphogen gradient of Bone Morphogenetic Protein signaling. Scale invariance of DV patterning by BMPs has been found in both vertebrates and invertebrates, however the mechanisms that regulate gradient scaling remain controversial. To obtain quantitative data that can be used to address core questions of scaling, we introduce a method to tune the size of zebrafish embryos by reducing varying amounts of vegetal yolk. We quantified the BMP signaling gradient in wild-type and perturbed embryos and found that the system scales for reductions in cross-sectional perimeter of up to 30%. Furthermore, we found that the degree of scaling for intraspecies scaling within zebrafish is greater than that between Danioninae species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping