PUBLICATION

Effect of MS222 on Hemostasis in Zebrafish

Authors
Deebani, A., Iyer, N., Raman, R., Jagadeeswaran, P.
ID
ZDB-PUB-190331-4
Date
2019
Source
Journal of the American Association for Laboratory Animal Science : JAALAS   58(3): 390-396 (Journal)
Registered Authors
Jagadeeswaran, Pudur
Keywords
none
MeSH Terms
  • Aminobenzoates/pharmacology*
  • Anesthesia
  • Anesthetics/pharmacology*
  • Animals
  • Blood Cell Count/veterinary
  • Blood Coagulation/drug effects
  • Hemostasis/drug effects*
  • Zebrafish/blood*
PubMed
30925946 Full text @ J. Am. Assoc. Lab. Anim. Sci.
Abstract
MS222 is a compound used in anesthetizing vertebrates, including fish and frogs. Several side effects of this anesthetichave been reported, but its effect on hemostasis has not been studied. In our laboratory, we have used zebrafish for morethan 2 decades as a model system to study hemostasis. During this period, we have had trouble in collecting blood from anesthetizedzebrafish and observed more rapid blood clotting than in nonanesthetized counterparts. However, no systematic studies regarding the effect of MS222 on zebrafish hemostasis are available. In this study, we performed various assays suchas gill bleeding, measurement of Hct, total blood cell counts, thrombocyte counts, thrombocyte aggregation, and coagulationand measured the amount of blood collected. We found that Hct values, the amount of blood collected, bleeding, andcoagulation differed significantly between anesthetized and nonanesthetized fish. Our results suggest that blood collectedafter MS222 anesthesia of zebrafish has altered hemostasis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping