PUBLICATION

Identification of Zebrafish Calcium Toolkit Genes and their Expression in the Brain

Authors
Wasilewska, I., Gupta, R.K., Palchevska, O., Kuźnicki, J.
ID
ZDB-PUB-190321-2
Date
2019
Source
Genes   10(3): (Journal)
Registered Authors
Keywords
RNA-sequencing, RT-PCR, bioinformatics, brain, calcium homeostasis, calcium signaling, calcium toolkit, gene ontology, neurons, zebrafish
Datasets
GEO:GSE127335
MeSH Terms
  • Animals
  • Brain/metabolism*
  • Calcium Signaling*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Models, Animal
  • Sequence Analysis, RNA
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
PubMed
30889933 Full text @ Genes (Basel)
Abstract
Zebrafish are well-suited for in vivo calcium imaging because of the transparency of their larvae and the ability to express calcium probes in various cell subtypes. This model organism has been used extensively to study brain development, neuronal function, and network activity. However, only a few studies have investigated calcium homeostasis and signaling in zebrafish neurons, and little is known about the proteins that are involved in these processes. Using bioinformatics analysis and available databases, the present study identified 491 genes of the zebrafish Calcium Toolkit (CaTK). Using RNA-sequencing, we then evaluated the expression of these genes in the adult zebrafish brain and found 380 hits that belonged to the CaTK. Based on quantitative real-time polymerase chain reaction arrays, we estimated the relative mRNA levels in the brain of CaTK genes at two developmental stages. In both 5 dpf larvae and adult zebrafish, the highest relative expression was observed for tmbim4, which encodes a Golgi membrane protein. The present data on CaTK genes will contribute to future applications of zebrafish as a model for in vivo and in vitro studies of Ca2+ signaling.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping