PUBLICATION

Pharmacological screening of a new alpha-2 adrenergic receptor agonist, mafedine, in zebrafish

Authors
Sysoev, Y.I., Meshalkina, D.A., Petrov, D.V., Okovityi, S.V., Musienko, P.E., Kalueff, A.V.
ID
ZDB-PUB-190306-17
Date
2019
Source
Neuroscience letters   701: 234-239 (Journal)
Registered Authors
Kalueff, Allan V.
Keywords
alpha-2 agonists, anxiogenic effects, mafedine, novel tank test, psychostimulant action, zebrafish
MeSH Terms
  • Adrenergic alpha-2 Receptor Agonists/pharmacology*
  • Animals
  • Behavior, Animal/drug effects*
  • Dose-Response Relationship, Drug
  • Drug Evaluation, Preclinical/methods
  • Mafenide/pharmacology*
  • Zebrafish
PubMed
30836120 Full text @ Neurosci. Lett.
Abstract
Pharmacological agents acting at alpha-2 adrenergic receptors are widely used in physiology and neuroscience research. Mounting evidence of their potential utility in clinical and experimental psychopharmacology, necessitates new models and novel model organisms for their screening. Here, we characterize behavioral effects of mafedine (6-oxo-1-phenyl-2- (phenylamino)-1,6-dihydropyrimidine-4-sodium olate), a novel drug with alpha-2 adrenergic receptor agonistic effects, in adult zebrafish (Danio rerio) novel tank test of anxiety and activity. Following an acute 20-min exposure, mafedine at 60 mg/L produced a mild psychostimulant action with some anxiogenic-like effects. Repeated acute 20-min/day administration of mafedine for 7 consecutive days at 1, 5 and 10 mg/L had a similar action on fish behavior as an acute exposure to 60 mg/L. Since mafedine demonstrated robust behavioral effects in zebrafish - a sensitive vertebrate aquatic model, it is likely that it may modulate rodent and human behavior as well. Thus, further studies are needed to explore this possibility in detail, and whether it may foster clinical application of mafedine and related alpha-2 adrenergic agents.
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