|ZFIN ID: ZDB-PUB-190227-22|
Fluid dynamics during bleb formation in migrating cells in vivo
Goudarzi, M., Boquet-Pujadas, A., Olivo-Marin, J.C., Raz, E.
|Source:||PLoS One 14: e0212699 (Journal)|
|Registered Authors:||Goudarzi, Mohammad, Raz, Erez|
|PubMed:||30807602 Full text @ PLoS One|
Goudarzi, M., Boquet-Pujadas, A., Olivo-Marin, J.C., Raz, E. (2019) Fluid dynamics during bleb formation in migrating cells in vivo. PLoS One. 14:e0212699.
ABSTRACTBlebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells results primarily from redistribution of material within the cell rather than being driven by flow of water from an external source.