PUBLICATION

Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport

Authors
Insinna, C., Lu, Q., Teixeira, I., Harned, A., Semler, E.M., Stauffer, J., Magidson, V., Tiwari, A., Kenworthy, A.K., Narayan, K., Westlake, C.J.
ID
ZDB-PUB-190127-14
Date
2019
Source
Nature communications   10: 428 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Adaptor Proteins, Signal Transducing/chemistry*
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/metabolism
  • Animals
  • Basal Bodies/metabolism*
  • Basal Bodies/ultrastructure
  • Biological Transport
  • Cell Line
  • Cell Membrane/metabolism
  • Cell Membrane/ultrastructure
  • Centrioles/metabolism
  • Centrioles/ultrastructure
  • Cilia/metabolism*
  • Cilia/ultrastructure
  • Embryo, Nonmammalian
  • Epithelial Cells/metabolism*
  • Epithelial Cells/ultrastructure
  • Gene Expression Regulation
  • Humans
  • Membrane Fusion
  • Mice
  • NIH 3T3 Cells
  • Protein Binding
  • Protein Domains
  • Signal Transduction
  • Vesicular Transport Proteins/genetics*
  • Vesicular Transport Proteins/metabolism
  • Zebrafish
PubMed
30683896 Full text @ Nat. Commun.
Abstract
The intracellular ciliogenesis pathway requires membrane trafficking, fusion, and reorganization. Here, we demonstrate in human cells and zebrafish that the F-BAR domain containing proteins PACSIN1 and -2 play an essential role in ciliogenesis, similar to their binding partner and membrane reorganizer EHD1. In mature cilia, PACSINs and EHDs are dynamically localized to the ciliary pocket membrane (CPM) and transported away from this structure on membrane tubules along with proteins that exit the cilium. PACSINs function early in ciliogenesis at the ciliary vesicle (CV) stage to promote mother centriole to basal body transition. Remarkably, we show that PACSIN1 and EHD1 assemble membrane t7ubules from the developing intracellular cilium that attach to the plasma membrane, creating an extracellular membrane channel (EMC) to the outside of the cell. Together, our work uncovers a function for F-BAR proteins and membrane tubulation in ciliogenesis and explains how the intracellular cilium emerges from the cell.
Errata / Notes
This article is corrected by ZDB-PUB-220906-138 .
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