PUBLICATION

Dynamic and non-contact 3D sample rotation for microscopy

Authors
Berndt, F., Shah, G., Power, R.M., Brugués, J., Huisken, J.
ID
ZDB-PUB-181130-18
Date
2018
Source
Nature communications   9: 5025 (Journal)
Registered Authors
Huisken, Jan
Keywords
none
MeSH Terms
none
PubMed
30487638 Full text @ Nat. Commun.
Abstract
Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.
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