PUBLICATION
Characterization of ADP-ribosyl cyclase 1-like (ARC1-like) activity and NAADP signaling during slow muscle cell development in zebrafish embryos
- Authors
- Kelu, J.J., Webb, S.E., Galione, A., Miller, A.L.
- ID
- ZDB-PUB-181118-20
- Date
- 2018
- Source
- Developmental Biology 445(2): 211-225 (Journal)
- Registered Authors
- Galione, Antony, Miller, Andrew L., Webb, Sarah E.
- Keywords
- ARC, Ca(2+) signaling, NAADP, Slow muscle cells, Zebrafish
- MeSH Terms
-
- ADP-ribosyl Cyclase 1/antagonists & inhibitors
- ADP-ribosyl Cyclase 1/genetics
- ADP-ribosyl Cyclase 1/metabolism*
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified
- Calcium Channels/metabolism
- Calcium Signaling
- Gene Knockdown Techniques
- Muscle Cells/metabolism
- Muscle Development
- NADP/analogs & derivatives*
- NADP/metabolism
- Niacinamide/pharmacology
- Sarcoplasmic Reticulum/metabolism
- Sequence Homology, Amino Acid
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 30447180 Full text @ Dev. Biol.
Citation
Kelu, J.J., Webb, S.E., Galione, A., Miller, A.L. (2018) Characterization of ADP-ribosyl cyclase 1-like (ARC1-like) activity and NAADP signaling during slow muscle cell development in zebrafish embryos. Developmental Biology. 445(2):211-225.
Abstract
We recently demonstrated the requirement of two-pore channel type 2 (TPC2)-mediated Ca2+ release during slow muscle cell differentiation and motor circuit maturation in intact zebrafish embryos. However, the upstream trigger of TPC2/Ca2+ signaling during these developmental processes remains unclear. Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent Ca2+ mobilizing messenger, which is suggested to target TPC2 in mediating the release of Ca2+ from acidic vesicles. Here, we report the molecular cloning of the zebrafish ADP ribosyl cyclase (ARC) homolog (i.e., ARC1-like), which is the putative enzyme for generating NAADP. We characterized the expression of the arc1-like transcript and the NAADP level between ~16hours post-fertilization (hpf) and ~48 hpf in whole zebrafish embryos. We showed that when ARC1-like was fused with either EGFP or tdTomato, it was localized in the plasma membrane, and associated with intracellular organelles, such as the acidic vesicles, Golgi complex and sarcoplasmic reticulum, in primary muscle cell cultures. Morpholino (MO)-mediated knockdown of arc1-like or pharmacological inhibition of ARC1 (via treatment with nicotinamide), led to an attenuation of Ca2+ signaling and disruption of slow muscle cell development. In addition, the injection of arc1-like mRNA into ARC1-like morphants partially rescued the Ca2+ signals and slow muscle cell development. Together, our data might suggest a link between ARC1-like, NAADP, TPC2 and Ca2+ signaling during zebrafish myogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping