PUBLICATION
Characterizing sources of variability in zebrafish embryo screening protocols
- Authors
- Hamm, J.T., Ceger, P., Allen, D., Stout, M., Maull, E.A., Baker, G., Zmarowski, A., Padilla, S., Perkins, E., Planchart, A., Stedman, D., Tal, T., Tanguay, R.L., Volz, D.C., Wilbanks, M.S., Walker, N.J.
- ID
- ZDB-PUB-181114-12
- Date
- 2018
- Source
- Altex 36(1): 103-120 (Journal)
- Registered Authors
- Tanguay, Robyn L.
- Keywords
- none
- MeSH Terms
-
- Animals
- Chorion/metabolism
- Drug Evaluation, Preclinical/methods
- Embryo, Nonmammalian*
- Embryonic Development/drug effects
- High-Throughput Screening Assays
- Toxicity Tests/methods*
- Zebrafish/embryology*
- PubMed
- 30415271 Full text @ Altex
Citation
Hamm, J.T., Ceger, P., Allen, D., Stout, M., Maull, E.A., Baker, G., Zmarowski, A., Padilla, S., Perkins, E., Planchart, A., Stedman, D., Tal, T., Tanguay, R.L., Volz, D.C., Wilbanks, M.S., Walker, N.J. (2018) Characterizing sources of variability in zebrafish embryo screening protocols. Altex. 36(1):103-120.
Abstract
There is a need for fast, efficient, and cost-effective hazard identification and characterization of chemical hazards. This need is generating increased interest in the use of zebrafish embryos as both a screening tool and an alternative to mammalian test methods. A Collaborative Workshop on Aquatic Models and 21st Century Toxicology identified the lack of appropriate and consistent testing protocols as a challenge to the broader application of the zebrafish embryo model. The National Toxicology Program established the Systematic Evaluation of the Application of Zebrafish in Toxicology (SEAZIT) initiative to address the lack of consistent testing guidelines and identify sources of variability for zebrafish-based assays. This report summarizes initial SEAZIT information-gathering efforts. Investigators in academic, government, and industry laboratories that routinely use zebrafish embryos for chemical toxicity testing were asked about their husbandry practices and standard protocols. Information was collected about protocol components including zebrafish strains, feed, system water, disease surveillance, embryo exposure conditions, and endpoints. Literature was reviewed to assess issues raised by the investigators. Interviews revealed substantial variability across design parameters, data collected, and analysis procedures. The presence of the chorion and renewal of exposure media (static versus static-renewal) were identified as design parameters that could potentially influence study outcomes and should be investigated further with studies to determine chemical uptake from treatment solution into embryos. The information gathered in this effort provides a basis for future SEAZIT activities to promote more consistent practices among researchers using zebrafish embryos for toxicity evaluation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping