PUBLICATION
Grk1b and Grk7a Both Contribute to the Recovery of the Isolated Cone Photoresponse in Larval Zebrafish
- Authors
- Chrispell, J.D., Dong, E., Osawa, S., Liu, J., Cameron, D.J., Weiss, E.R.
- ID
- ZDB-PUB-181030-6
- Date
- 2018
- Source
- Investigative ophthalmology & visual science 59: 5116-5124 (Journal)
- Registered Authors
- Chrispell, Jared, Liu, Jiandong, Weiss, Ellen R.
- Keywords
- none
- MeSH Terms
-
- Animals
- Contrast Sensitivity/physiology
- Dark Adaptation
- Electroretinography
- Fluorescent Antibody Technique, Indirect
- G-Protein-Coupled Receptor Kinase 1/physiology*
- G-Protein-Coupled Receptor Kinases/physiology*
- Gene Silencing/physiology
- Larva
- Nystagmus, Optokinetic/physiology
- Phosphorylation
- Photic Stimulation
- Recovery of Function/physiology*
- Retinal Cone Photoreceptor Cells/physiology*
- Vision, Ocular
- Visual Acuity/physiology
- Zebrafish
- Zebrafish Proteins/physiology*
- PubMed
- 30372740 Full text @ Invest. Ophthalmol. Vis. Sci.
Citation
Chrispell, J.D., Dong, E., Osawa, S., Liu, J., Cameron, D.J., Weiss, E.R. (2018) Grk1b and Grk7a Both Contribute to the Recovery of the Isolated Cone Photoresponse in Larval Zebrafish. Investigative ophthalmology & visual science. 59:5116-5124.
Abstract
Purpose To define the functional roles of Grk1 and Grk7 in zebrafish cones in vivo.
Methods Genome editing was used to generate grk7a and grk1b knockout zebrafish. Electroretinogram (ERG) analyses of the isolated cone mass receptor potential and the b-wave were performed in dark-adapted zebrafish using a paired flash paradigm to determine recovery of cone photoreceptors and the inner retina after an initial flash. In addition, psychophysical visual response was measured using the optokinetic response (OKR).
Results ERG analysis demonstrated that deletion of either Grk1b or Grk7a in zebrafish larvae resulted in modestly lower rates of recovery of the isolated cone mass receptor potential from an initial flash compared to wildtype larvae. On the other hand, grk1b-/- and grk7a-/- larvae exhibited a b-wave recovery that was similar to wildtype larvae. We evaluated the OKR and found that deletion of either Grk1b or Grk7a leads to a small decrease in temporal contrast sensitivity and alterations in visual acuity.
Conclusions For the first time, we demonstrate that Grk1b and Grk7a both contribute to visual function in larval zebrafish cones. Since the difference between wildtype and each knockout fish is modest, it appears that either GRK is sufficient for adequate cone visual function.
Errata / Notes
This article is corrected by ZDB-PUB-220906-127 .
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping