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ZFIN ID: ZDB-PUB-181020-2
Sandensolide Induces Oxidative Stress-Mediated Apoptosis in Oral Cancer Cells and in Zebrafish Xenograft Model
Yu, C.I., Chen, C.Y., Liu, W., Chang, P.C., Huang, C.W., Han, K.F., Lin, I.P., Lin, M.Y., Lee, C.H.
Date: 2018
Source: Marine drugs   16(10): (Journal)
Registered Authors:
Keywords: human oral squamous cell carcinoma, reactive oxygen species, sandensolide
MeSH Terms:
  • Acetylcysteine/pharmacology
  • Animals
  • Anthozoa*
  • Antineoplastic Agents/isolation & purification
  • Antineoplastic Agents/pharmacology*
  • Antineoplastic Agents/therapeutic use
  • Apoptosis/drug effects
  • Carcinoma, Squamous Cell/drug therapy*
  • Cell Cycle Checkpoints/drug effects
  • Cell Line, Tumor
  • Cell Proliferation/drug effects
  • Diterpenes/chemistry
  • Diterpenes/isolation & purification
  • Diterpenes/pharmacology*
  • Diterpenes/therapeutic use
  • Humans
  • Mouth Neoplasms/drug therapy*
  • Oxidative Stress/drug effects
  • Reactive Oxygen Species/metabolism
  • Xenograft Model Antitumor Assays
  • Zebrafish
PubMed: 30332851 Full text @ Mar. Drugs
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ABSTRACT
Presently, natural sources and herbs are being sought for the treatment of human oral squamous cell carcinoma (OSCC) in order to alleviate the side effects of chemotherapy. This study investigates the effect of sandensolide, a cembrane isolated from Sinularia flexibilis, to inhibit human OSCC cell growth with the aim of developing a new drug for the treatment of oral cancer. In vitro cultured human OSCC models (Ca9.22, SCC9 and HSC-3 cell lines) and oral normal cells (HGF-1), as well as a zebrafish xenograft model, were used to test the cytotoxicity of sandensolide (MTT assay), as well as to perform cell cycle analysis and Western blotting. Both the in vitro bioassay and the zebrafish xenograft model demonstrated the anti-oral cancer effect of sandensolide. Moreover, sandensolide was able to significantly suppress colony formation and induce apoptosis, as well as cell cycle arrest, in OSCC by regulating multiple key proteins. Induction of reactive oxygen species (ROS) was observed in sandensolide-treated oral cancer cells. However, these apoptotic changes were rescued by NAC pretreatment. These findings contribute to the knowledge of the model of action of sandensolide, which may induce oxidative stress-mediated cell death pathways as a potential agent in oral cancer therapeutics.
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