PUBLICATION

Environmentally relevant concentration of chromium activates Nrf2 and alters transcription of related XME genes in liver of zebrafish

Authors
Shaw, P., Mondal, P., Bandyopadhyay, A., Chattopadhyay, A.
ID
ZDB-PUB-180927-11
Date
2018
Source
Chemosphere   214: 35-46 (Journal)
Registered Authors
Keywords
Chromium, Environmentally relevant, Hepatotoxicity, Nrf2, Oxidative stress, Zebrafish
MeSH Terms
  • Animals
  • Chromium/adverse effects*
  • Chromium/chemistry
  • Fishes
  • Liver/chemistry*
  • Liver/pathology
  • NF-E2-Related Factor 2/genetics*
  • Oxidative Stress
  • Zebrafish
PubMed
30253254 Full text @ Chemosphere
Abstract
Fish is an excellent model to decipher the mechanism of toxicity of aquatic contaminants such as hexavalent chromium (Cr [VI]). The present study looked into the manifestation of stress in liver of zebrafish exposed to an environmentally relevant concentration (2 mgL-1), and the functioning of the cytoprotective machinery that pacifies the formed stress. The results lead us to hypothesize that oxidative stress plays a key role in chromium-induced toxicity resulting in lipid peroxidation and extensive changes in tissue ultrastructure. In treated fish, production of reactive oxygen species, increase in reduced glutathione content and increase in malondialdehyde content along with enhanced catalase activity were evident. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) was found to increase both at transcriptional and translational level and its translocation into the nucleus was confirmed by fluorescence-based immunohistochemical studies. The mRNA levels of genes like Nqo1, Cyp1a and Cu/Zn Sod were found to increase whereas Ho1, Hsp70 and Ucp2 were down-regulated. The sensitivity of these genes towards Cr [VI] validates their candidature as important biomarkers of Cr [VI] exposure in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping