PUBLICATION
Polydatin alleviated alcoholic liver injury in zebrafish larvae through ameliorating lipid metabolism and oxidative stress
- Authors
- Lai, Y., Zhou, C., Huang, P., Dong, Z., Mo, C., Xie, L., Lin, H., Zhou, Z., Deng, G., Liu, Y., Chen, Y., Huang, S., Wu, Z., Sun, X., Gao, L., Lv, Z.
- ID
- ZDB-PUB-180925-4
- Date
- 2018
- Source
- Journal of Pharmacological Sciences 138(1): 46-53 (Journal)
- Registered Authors
- Keywords
- Alcoholic liver disease, Lipid metabolism, Oxidative stress, Polydatin, Zebrafish
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents*
- Antioxidants*
- Aryl Hydrocarbon Hydroxylases/genetics
- Aryl Hydrocarbon Hydroxylases/metabolism
- Cell Cycle Proteins/genetics
- Cell Cycle Proteins/metabolism
- Cytochrome P450 Family 3/genetics
- Cytochrome P450 Family 3/metabolism
- DNA Damage/genetics
- Fallopia japonica/chemistry
- Gene Expression/drug effects
- Glucosides/isolation & purification
- Glucosides/pharmacology*
- Glucosides/therapeutic use
- Lipid Metabolism/drug effects*
- Lipid Metabolism/genetics
- Liver Diseases, Alcoholic/drug therapy*
- Liver Diseases, Alcoholic/genetics
- Liver Diseases, Alcoholic/metabolism*
- Liver Diseases, Alcoholic/pathology
- Nuclear Proteins/genetics
- Nuclear Proteins/metabolism
- Oxidative Stress/drug effects*
- Oxidoreductases, N-Demethylating/genetics
- Oxidoreductases, N-Demethylating/metabolism
- Phytotherapy*
- Stilbenes/isolation & purification
- Stilbenes/pharmacology*
- Stilbenes/therapeutic use
- Zebrafish*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 30245287 Full text @ J. Pharmacol. Sci.
Citation
Lai, Y., Zhou, C., Huang, P., Dong, Z., Mo, C., Xie, L., Lin, H., Zhou, Z., Deng, G., Liu, Y., Chen, Y., Huang, S., Wu, Z., Sun, X., Gao, L., Lv, Z. (2018) Polydatin alleviated alcoholic liver injury in zebrafish larvae through ameliorating lipid metabolism and oxidative stress. Journal of Pharmacological Sciences. 138(1):46-53.
Abstract
Hepatic steatosis is the early stage of alcoholic liver disease (ALD), may progress to steatohepatitis, fibrosis even cirrhosis. Polydatin, the primary active component of Polygonum cuspidatum Sieb. et Zucc, has been recognized to possess hepatoprotective and anti-inflammatory properties. To investigate whether polydatin alleviates ethanol induced liver injury and to elucidate the underlying molecular mechanisms, zebrafish larvae at 4 days post-fertilization (dpf) were exposed to 350 mmol/L of ethanol for 32 h, then treated with polydatin for 48 h. Oil red O, Nile Red and H&E staining were used to analyze the pathological changes in liver. The mRNA levels were measured by quantitative PCR and the antioxidant capacity was detected using H2O2-specific fluorescent probe. Here, polydatin strongly alleviated hepatic steatosis and decreased the expression levels of alcohol and lipid metabolism-related genes, including CYP2Y3, CYP3A65, HMGCRa, HMGCRb and FASN. Additionally, polydatin inhibited oxidative stress in the liver according to fluorescent probe. Moreover, significantly up-regulated expression of DNA damage-related genes (CHOP, GADD45αa) revealed that polydatin attenuated hepatic apoptosis in larvae. In conclusion, polydatin may improve the liver function of zebrafish with acute alcoholic liver injury through attenuating hepatic fat accumulation, ameliorating lipid and ethanol metabolism and reducing oxidative stress and DNA damage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping