PUBLICATION
Distinct requirements of E2f4 versus E2f5 activity for multiciliated cell development in the zebrafish embryo
- Authors
- Chong, Y.L., Zhang, Y., Zhou, F., Roy, S.
- ID
- ZDB-PUB-180916-6
- Date
- 2018
- Source
- Developmental Biology 443(2): 165-172 (Journal)
- Registered Authors
- Roy, Sudipto
- Keywords
- E2f4, E2f5, Gmnc, Multicilin, cilia, multiciliated cell, zebrafish
- MeSH Terms
-
- Animals
- Cell Cycle Proteins/metabolism
- Cell Differentiation/genetics
- Cilia/metabolism
- Cilia/physiology
- E2F4 Transcription Factor/metabolism*
- E2F4 Transcription Factor/physiology
- E2F5 Transcription Factor/metabolism*
- E2F5 Transcription Factor/physiology
- Gene Expression Regulation, Developmental/genetics
- HEK293 Cells
- Humans
- Nuclear Proteins/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 30218642 Full text @ Dev. Biol.
Citation
Chong, Y.L., Zhang, Y., Zhou, F., Roy, S. (2018) Distinct requirements of E2f4 versus E2f5 activity for multiciliated cell development in the zebrafish embryo. Developmental Biology. 443(2):165-172.
Abstract
Multiciliated cells (MCCs) differentiate arrays of motile cilia that beat to drive fluid flow over epithelia. Recent studies have established two Geminin family coiled-coil containing nuclear regulatory proteins, Gmnc and Multicilin (Mci), in the specification and differentiation of the MCCs. Both Gmnc and Mci are devoid of a DNA binding domain: they regulate transcription by associating with E2f family transcription factors, notably E2f4 and E2f5. Here, we have studied the relative contribution of these two E2f factors in MCC development using the zebrafish embryo, which differentiates MCCs within kidney tubules and the nose. We found that while E2f4 is fully dispensable, E2f5 is essential for MCCs to form in the kidney tubules. Moreover, using a variety of double mutant combinations we show that E2f5 has a more prominent role in MCC development in the zebrafish than E2f4. This contrasts with current evidence from the mouse, where E2f4 seems to be more important. Thus, distinct combinatorial activities of the E2f4 and E2f5 proteins regulate the specification and differentiation of MCCs in zebrafish and mice.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping