PUBLICATION
A myeloid differentiation factor 88 gene from yellow catfish Pelteobagrus fulvidraco and its molecular characterization in response to polyriboinosinic polyribocytidylic acid and lipopolysaccharide challenge
- Authors
- Zhao, X.M., Chu, X.H., Liu, Y., Liu, Q.N., Jiang, S.H., Zhang, D.Z., Tang, B.P., Zhou, C.L., Dai, L.S.
- ID
- ZDB-PUB-180904-7
- Date
- 2018
- Source
- International journal of biological macromolecules 120(Pt A): 1080-1086 (Journal)
- Registered Authors
- Keywords
- Expression analysis, Immune response, Myeloid differentiation factor 88
- MeSH Terms
-
- Amino Acid Sequence/genetics
- Animals
- Catfishes/genetics*
- Cloning, Molecular
- Lipopolysaccharides/pharmacology
- Myeloid Differentiation Factor 88/chemistry
- Myeloid Differentiation Factor 88/genetics*
- Phylogeny*
- Poly I-C/pharmacology
- Tissue Distribution/drug effects
- PubMed
- 30176326 Full text @ Int. J. Biol. Macromol.
Citation
Zhao, X.M., Chu, X.H., Liu, Y., Liu, Q.N., Jiang, S.H., Zhang, D.Z., Tang, B.P., Zhou, C.L., Dai, L.S. (2018) A myeloid differentiation factor 88 gene from yellow catfish Pelteobagrus fulvidraco and its molecular characterization in response to polyriboinosinic polyribocytidylic acid and lipopolysaccharide challenge. International journal of biological macromolecules. 120(Pt A):1080-1086.
Abstract
Myeloid differentiation factor 88 (MyD88) is an adaptor protein of Toll-like receptor (TLR) signalling pathways that activates the innate immune system. Herein, MyD88 was identified in the economically important freshwater fish Pelteobagrus fulvidraco. The complete 2156 bp PfMyD88 cDNA includes a 147 bp 5'-untranslated region (UTR), a 1133 bp 3'-UTR, and an open reading frame (ORF) of 876 bp encoding a 291 residue protein containing Death and Toll/interleukin-1 receptor (TIR) domains. The deduced protein sequence shares 88.8%, 73.8% and 59.3% identity with orthologs in Ictalurus punctatus, Danio rerio and Homo sapiens, respectively. qRT-PCR revealed expression in all tested tissues, highest in trunk kidney, followed by spleen, and lowest in muscle. After challenge with lipopolysaccharide (LPS) or polyriboinosinic polyribocytidylic acid (Poly I:C), PfMyD88 expression was up-regulated in blood, liver, head kidney and spleen. Thus, PfMyD88 acts in innate immunity in P. fulvidraco.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping