PUBLICATION

Segmentation pattern of zebrafish caudal fin is affected by developmental temperature and defined by multiple fusions between segments

Authors
Christou, M., Iliopoulou, M., Witten, P.E., Koumoundouros, G.
ID
ZDB-PUB-180830-8
Date
2018
Source
Journal of experimental zoology. Part B, Molecular and developmental evolution   330(6-7): 330-340 (Journal)
Registered Authors
Koumoundouros, Giorgos, Witten, P. Eckhard
Keywords
joints, lepidotrichia, phenotypic plasticity, segments, teleosts
MeSH Terms
  • Animal Fins/embryology*
  • Animal Fins/growth & development*
  • Animal Fins/physiology
  • Animals
  • Body Patterning
  • Bone Development/physiology
  • Bone and Bones/embryology
  • Bone and Bones/physiology
  • Embryo, Nonmammalian/physiology
  • Larva/growth & development
  • Larva/physiology
  • Temperature*
  • Zebrafish/embryology*
  • Zebrafish/growth & development*
  • Zebrafish/physiology
PubMed
30156749 Full text @ J. Exp. Zool. B Mol. Dev. Evol.
Abstract
Caudal-fin lepidotrichia is composed of numerous segments, which are linked to each other by intersegmental joints. During fish growth, lepidotrichia elongate by the addition of new segments at their distal margin, whereas the length of each segment remains constant after it is formed. In the present paper, we examined whether the water temperature affects the segmentation pattern of the juvenile and adult caudal fin. For this purpose, zebrafish (Danio rerio) embryos and larvae were exposed to three different temperature conditions (24°C, 28°C, and 32°C) from the pharyngula stage (1 day postfertilization [dpf]) to metamorphosis, whereas the control temperature (28°C) was applied to all the groups before and after this period. Results demonstrated that water temperature had a significant effect on the length of the segments of each lepidotrichium, at both the juvenile and adult stages. Moreover, at higher temperatures, there was a significant proximal shift of the position of the first bifurcation of the second lepidotrichium of the dorsal lobe. At all the experimental conditions, the length of proximal segment was not constant during fish growth, but it followed a discontinuous saltatory growth. Histological analysis of the proximal lepidotrichia segments revealed that the observed apparent growth of segments is the result of fusions between segments. Fusion occurs not by mineralization of the intersegmental joints, but by bone deposition around the joints.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping