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ZIRC
ZFIN ID: ZDB-PUB-180805-5
Programmable base editing in zebrafish using a modified CRISPR-Cas9 system
Qin, W., Lu, X., Lin, S.
Date: 2018
Source: Methods (San Diego, Calif.)   150: 19-23 (Journal)
Registered Authors: Lin, Shuo
Keywords: none
MeSH Terms:
  • Abnormalities, Multiple/genetics
  • Animals
  • Base Sequence/genetics
  • CRISPR-Cas Systems/genetics*
  • Cytidine/metabolism
  • Cytidine Deaminase/genetics
  • Disease Models, Animal
  • Embryo, Nonmammalian
  • Eye Abnormalities/genetics
  • Female
  • Gene Editing/methods*
  • Humans
  • Macrostomia/genetics
  • Male
  • Mutagenesis, Site-Directed/methods
  • Point Mutation/genetics
  • RNA, Guide
  • Thymine/metabolism
  • Twist-Related Protein 2/genetics
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
PubMed: 30076894 Full text @ Methods
FIGURES
ABSTRACT
The use of CRISPR/Cas9 to knockout genes in zebrafish has been well established. However, to better model many human diseases that are caused by point mutations, a robust methodology for generating desirable DNA base changes is still needed. Recently, Cas9-linked cytidine deaminases (base editors) evolved as a strategy to introduce single base mutations in model organisms. They have been used to convert cytidine to thymine at specific genomic loci. Here we describe a protocol for using the base editing system in zebrafish and its application to reproduce a single base mutation observed in human Ablepharon-Macrostomia Syndrome.
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