PUBLICATION
Silver nanoparticles affect lens rather than retina development in zebrafish embryos
- Authors
- Zhang, Y., Wang, Z., Zhao, G., Liu, J.X.
- ID
- ZDB-PUB-180730-4
- Date
- 2018
- Source
- Ecotoxicology and environmental safety 163: 279-288 (Journal)
- Registered Authors
- Liu, Jing-xia
- Keywords
- AgNPs, Embryos, Lens, Retina, Zerbrafish
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects*
- Embryonic Development/drug effects
- Lens, Crystalline/abnormalities
- Lens, Crystalline/drug effects*
- Lens, Crystalline/growth & development
- Metal Nanoparticles/toxicity*
- Retina/drug effects*
- Retina/growth & development
- Silver/toxicity*
- Zebrafish/abnormalities*
- PubMed
- 30056342 Full text @ Ecotoxicol. Environ. Saf.
Citation
Zhang, Y., Wang, Z., Zhao, G., Liu, J.X. (2018) Silver nanoparticles affect lens rather than retina development in zebrafish embryos. Ecotoxicology and environmental safety. 163:279-288.
Abstract
Silver nanoparticles (AgNPs) have been reported to inhibit specification and differentiation of erythroid cells, chromatophores, and myofibrils during zebrafish embryogenesis. However, the knowledge of biological effects of AgNPs on eye development, especially on lens development is scarce. In this study, embryos were exposed to or injected with 0.4 mg/L AgNPs, and the results indicate that no obvious morphological changes in eye formation were observed in the stressed embryos compared to the controls. However, clefts and vacuoles were observed in lens of embryos from AgNPs stressed group. Additionally, the down-regulated expressions of different lens crystallin isoform genes and the normal expression of retinal genes were observed in AgNPs stressed embryos, suggesting AgNPs might inhibit the development of lens rather than the development of retina in zebrafish embryos. Moreover, no obvious cell apoptosis was observed, but normal nuclear DNA and RNA export was observed in lens cells. Together, the data in this study reveal that AgNPs damage the development of lens rather than retina resulting in eye abnormalities via some unknown mechanisms rather than via triggering cells apoptosis or blocking nuclear DNA or RNA export.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping