ZFIN ID: ZDB-PUB-180502-18
Candidate gene identification of ovulation-inducing genes by RNA sequencing with an in vivo assay in zebrafish
Klangnurak, W., Fukuyo, T., Rezanujjaman, M.D., Seki, M., Sugano, S., Suzuki, Y., Tokumoto, T.
Date: 2018
Source: PLoS One   13: e0196544 (Journal)
Registered Authors: Fukuyo, Taketo, Tokumoto, Toshinobu
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Female
  • Gene Editing
  • Gene Knockout Techniques
  • Genomics
  • Ovulation/genetics*
  • Phenotype
  • Sequence Analysis, RNA*
  • Zebrafish/genetics*
  • Zebrafish/physiology*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
PubMed: 29715317 Full text @ PLoS One
We previously reported the microarray-based selection of three ovulation-related genes in zebrafish. We used a different selection method in this study, RNA sequencing analysis. An additional eight up-regulated candidates were found as specifically up-regulated genes in ovulation-induced samples. Changes in gene expression were confirmed by qPCR analysis. Furthermore, up-regulation prior to ovulation during natural spawning was verified in samples from natural pairing. Gene knock-out zebrafish strains of one of the candidates, the starmaker gene (stm), were established by CRISPR genome editing techniques. Unexpectedly, homozygous mutants were fertile and could spawn eggs. However, a high percentage of unfertilized eggs and abnormal embryos were produced from these homozygous females. The results suggest that the stm gene is necessary for fertilization. In this study, we selected additional ovulation-inducing candidate genes, and a novel function of the stm gene was investigated.