PUBLICATION
Stimulatory function of peroxiredoxin 1 in activating adaptive humoral immunity in a zebrafish model
- Authors
- Liu, G.P., Xiang, L.X., Shao, T., Lin, A.F., Shao, J.Z.
- ID
- ZDB-PUB-180309-8
- Date
- 2018
- Source
- Developmental and comparative immunology 84: 353-360 (Journal)
- Registered Authors
- Keywords
- Fish disease, Immune regulatory function, Initiation of adaptive immunity, Molecular adjuvant, Peroxiredoxin 1 (Prdx1)
- MeSH Terms
-
- Adaptive Immunity
- Animals
- B-Lymphocytes/immunology*
- CD4-Positive T-Lymphocytes/immunology*
- Cytokines/metabolism
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Histocompatibility Antigens Class II/metabolism
- Immunization
- Immunoglobulin M/metabolism
- Immunologic Memory
- Inflammation Mediators/metabolism
- Lymphocyte Activation
- Models, Animal
- NF-kappa B/metabolism
- Peroxiredoxins/genetics
- Peroxiredoxins/metabolism*
- Signal Transduction
- Toll-Like Receptor 4/metabolism
- Zebrafish/immunology*
- PubMed
- 29518406 Full text @ Dev. Comp. Immunol.
Citation
Liu, G.P., Xiang, L.X., Shao, T., Lin, A.F., Shao, J.Z. (2018) Stimulatory function of peroxiredoxin 1 in activating adaptive humoral immunity in a zebrafish model. Developmental and comparative immunology. 84:353-360.
Abstract
Peroxiredoxin 1 (Prdx1/Prx1), a ubiquitous antioxidant enzyme involved in preventing oxidative damage and maintaining redox homeostasis, is essential for various cellular activities. Extracellular Prdx1 also plays important roles in innate immune responses. However, little is known about the regulatory functions of Prdx1 in adaptive immunity. In the present study, we report the stimulatory role of Prdx1 in the initiation of adaptive humoral immunity in a zebrafish model. Administration of Prdx1 protein to zebrafish significantly induced the expression of TNF-α, IL-1β, and IL-6 by the Toll-like-receptor-4a-mediated NF-κB signaling pathway and enhanced the activation of MHC-II+ antigen-presenting cells, CD4+ T cells, and mIgM+ B cells. Subsequently, increased production of antigen-specific IgM antibody was observed. Thus, Prdx1 can be used as a novel molecular adjuvant with great therapeutic value for the vaccination of fish diseases. Our study improved the understanding of the biology of Prdx1 family.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping