PUBLICATION
Transcriptional activation of zebrafish fads2 promoter and its transient transgene expression in yolk syncytial layer of zebrafish embryos
- Authors
- Tay, S.S., Kuah, M.K., Shu-Chien, A.C.
- ID
- ZDB-PUB-180303-6
- Date
- 2018
- Source
- Scientific Reports 8: 3874 (Journal)
- Registered Authors
- Chong, Shu-Chien A.
- Keywords
- none
- MeSH Terms
-
- Animals
- Binding Sites
- Cloning, Molecular/methods
- Egg Yolk/metabolism
- Fatty Acid Desaturases/genetics*
- Fatty Acid Desaturases/metabolism
- Fatty Acids/genetics
- Fatty Acids/metabolism
- Fatty Acids, Unsaturated/biosynthesis
- Fatty Acids, Unsaturated/genetics
- Fish Proteins/genetics
- Gene Expression Regulation, Developmental
- Genes, Reporter
- Giant Cells/metabolism
- Promoter Regions, Genetic
- Transcriptional Activation
- Transgenes
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 29497119 Full text @ Sci. Rep.
Citation
Tay, S.S., Kuah, M.K., Shu-Chien, A.C. (2018) Transcriptional activation of zebrafish fads2 promoter and its transient transgene expression in yolk syncytial layer of zebrafish embryos. Scientific Reports. 8:3874.
Abstract
The front-end desaturases (Fads) are rate-limiting enzymes responsible for production of long-chain polyunsaturated fatty acids (LC-PUFA). The full spectrum of the transcriptional regulation of fads is still incomplete, as cloning of fads promoter is limited to a few species. Here, we described the cloning and characterisation of the zebrafish fads2 promoter. Using 5'-deletion and mutation analysis on this promoter, we identified a specific region containing the sterol regulatory element (SRE) which is responsible for the activation of the fads2 promoter. In tandem, two conserved CCAAT boxes were also present adjacent to the SRE and mutation of either of these binding sites attenuates the transcriptional activation of the fads2 promoter. An in vivo analysis employing GFP reporter gene in transiently transfected zebrafish embryos showed that this 1754 bp upstream region of the fads2 gene specifically directs GFP expression in the yolk syncytial layer (YSL) region. This indicates a role for LC-PUFA in the transport of yolk lipids through this tissue layer. In conclusion, besides identifying novel core elements for transcriptional activation in zebrafish fads2 promoter, we also reveal a potential role for fads2 or LC-PUFA in YSL during development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping