Making Waves: New Developments in Toxicology with the Zebrafish
- Horzmann, K.A., Freeman, J.L.
- Toxicological sciences : an official journal of the Society of Toxicology 163(1): 5-12 (Review)
- Registered Authors
- Freeman, Jennifer
- MeSH Terms
- Behavior, Animal/drug effects
- Embryo, Nonmammalian/drug effects
- Embryonic Development/drug effects
- High-Throughput Screening Assays
- Larva/drug effects
- Models, Animal*
- 29471431 Full text @ Toxicol. Sci.
Horzmann, K.A., Freeman, J.L. (2018) Making Waves: New Developments in Toxicology with the Zebrafish. Toxicological sciences : an official journal of the Society of Toxicology. 163(1):5-12.
The laboratory zebrafish (Danio rerio) is now an accepted model in toxicologic research. The zebrafish model fills a niche between in vitro models and mammalian biomedical models. The developmental characteristics of the small fish are strategically being used by scientists to study topics ranging from high-throughput toxicity screens to toxicity in multi- and transgenerational studies. High-throughput technology has increased the utility of zebrafish embryonic toxicity assays in screening of chemicals and drugs for toxicity or effect. Additionally, advances in behavioral characterization and experimental methodology allow for observation of recognizable phenotypic changes after xenobiotic exposure. Future directions in zebrafish research are predicted to take advantage of CRISPR-Cas9 genome editing methods in creating models of disease and interrogating mechanisms of action with fluorescent reporters or tagged proteins. Zebrafish can also model developmental origins of health and disease and multi- and transgenerational toxicity. The zebrafish has many advantages as a toxicologic model and new methodologies and areas of study continue to expand the usefulness and application of the zebrafish.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes