PUBLICATION
Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish
- Authors
- Paravani, E.V., Simoniello, M.F., Poletta, G.L., Zolessi, F.R., Casco, V.H.
- ID
- ZDB-PUB-180208-10
- Date
- 2018
- Source
- Mutation research 826: 25-32 (Journal)
- Registered Authors
- Zolessi, Flavio
- Keywords
- Apoptosis, DNA damage
- MeSH Terms
-
- Animals
- Caspase 3/metabolism
- Cell Survival
- DNA Damage*
- Dose-Response Relationship, Drug
- Histones/metabolism
- Hydrogen Peroxide/adverse effects
- Mutagenicity Tests
- Oxidative Stress*
- Photoreceptor Cells, Vertebrate/cytology*
- Photoreceptor Cells, Vertebrate/drug effects
- Photoreceptor Cells, Vertebrate/metabolism
- Pyrethrins/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish
- PubMed
- 29412866 Full text @ Mutat. Res.
Citation
Paravani, E.V., Simoniello, M.F., Poletta, G.L., Zolessi, F.R., Casco, V.H. (2018) Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish. Mutation research. 826:25-32.
Abstract
Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminants in freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD) and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show the presence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.6 μg/L CM. Histone γ-H2AX, a marker of DNA damage, was detected in both outer and inner nuclear layers; caspase-3, an apoptotic marker, was detected in the outer nuclear layer. In the comet assay, the cells were sensitive to hydrogen peroxide-induced DNA damage, showing a dose-dependent response. We observed a positive comet assay response to CM that was dose- and time-dependent. Following exposure to CM, SOD and CAT enzyme activities, and sod and cat mRNA levels, increased. These results indicate that CM causes DNA damage and oxidative stress and can induce apoptosis in retinal cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping