PUBLICATION
Methylparaben induces malformations and alterations on apoptosis, oxidant-antioxidant status, ccnd1 and myca expressions in zebrafish embryos
- Authors
- Ateş, P.S., Ünal, İ., Üstündağ, Ü.V., Alturfan, A.A., Yiğitbaşı, T., Emekli-Alturfan, E.
- ID
- ZDB-PUB-180124-5
- Date
- 2018
- Source
- Journal of biochemical and molecular toxicology 32(3): e22036 (Journal)
- Registered Authors
- Ateş, Perihan Seda, Emekli-Alturfan, Ebru, Unal, Ismail, Üstündağ, Ünsal Veli
- Keywords
- apoptosis, ccnd1, methylparaben, myca, oxidant-antioxidant status
- MeSH Terms
-
- Abnormalities, Multiple/chemically induced*
- Abnormalities, Multiple/metabolism
- Abnormalities, Multiple/pathology
- Animals
- Apoptosis/drug effects*
- Cyclin D1/biosynthesis*
- Gene Expression Regulation/drug effects*
- Parabens/toxicity*
- Proto-Oncogene Proteins c-myc/biosynthesis*
- Proto-Oncogene Proteins c-myc/blood
- Zebrafish/metabolism*
- Zebrafish Proteins/biosynthesis*
- PubMed
- 29360218 Full text @ J. Biochem. Mol. Toxicol.
- CTD
- 29360218
Citation
Ateş, P.S., Ünal, İ., Üstündağ, Ü.V., Alturfan, A.A., Yiğitbaşı, T., Emekli-Alturfan, E. (2018) Methylparaben induces malformations and alterations on apoptosis, oxidant-antioxidant status, ccnd1 and myca expressions in zebrafish embryos. Journal of biochemical and molecular toxicology. 32(3):e22036.
Abstract
Methylparabens (MP) are widely used as preservatives in cosmetics, pharmacy, and food industry. Although acute toxicity studies in animals indicated that parabens are not significantly toxic, the effects of chronic exposure under sublethal doses are still unknown and the number of related studies is limited. Our aim was to evaluate the effects of MP on the development of zebrafish embryos focusing on development, locomotor activity, oxidant-antioxidant status, apoptosis, and ccnd1 and myca expressions. The expressions of ccnd1 and myca were determined by RT-PCR. Lipid peroxidation (LPO), nitric oxide (NO), and glutathione-S-transferase (GST) activities were determined spectrophotometrically. Apoptosis was determined using acridine orange staining. Locomotor activity was measured using touch-evoked movement test. MP exposure increased malformations, LPO, apoptosis, ccnd1 and myca expressions, and decreased GST activities and NO levels compared with the control group. Our findings will lead to further understanding of the mechanism of MP toxicity, and merit further research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping