PUBLICATION
Patient-derived xenograft in zebrafish embryos: a new platform for translational research in gastric cancer
- Authors
- Wu, J.Q., Zhai, J., Li, C.Y., Tan, A.M., Wei, P., Shen, L.Z., He, M.F.
- ID
- ZDB-PUB-171117-11
- Date
- 2017
- Source
- Journal of experimental & clinical cancer research : CR 36: 160 (Journal)
- Registered Authors
- Keywords
- Gastric cancer, Microinjection, Patient-derived xenograft, Translational research, Zebrafish
- MeSH Terms
-
- Aged
- Animals
- Antineoplastic Agents/administration & dosage*
- Antineoplastic Agents/pharmacology
- Cell Line, Tumor
- Cell Proliferation/drug effects
- Cell Survival/drug effects
- China
- Disease Models, Animal
- Female
- Fluorouracil/administration & dosage
- Fluorouracil/pharmacology
- Humans
- Male
- Microinjections
- Middle Aged
- Neoplasm Invasiveness
- Neoplasm Transplantation/methods*
- Pyridines/administration & dosage
- Pyridines/pharmacology
- Stomach Neoplasms/drug therapy*
- Stomach Neoplasms/pathology*
- Taxoids/administration & dosage
- Taxoids/pharmacology
- Translational Research, Biomedical
- Treatment Outcome
- Zebrafish/embryology*
- PubMed
- 29141689 Full text @ J. Exp. Clin. Cancer Res.
Citation
Wu, J.Q., Zhai, J., Li, C.Y., Tan, A.M., Wei, P., Shen, L.Z., He, M.F. (2017) Patient-derived xenograft in zebrafish embryos: a new platform for translational research in gastric cancer. Journal of experimental & clinical cancer research : CR. 36:160.
Abstract
Background Gastric cancer (GC) is among the most commonly cancer occurred in Asian, especially in China. With its high heterogeneity and few of validated drug targets, GC remains to be one of the most under explored areas of precision medicine. In this study, we aimed to establish an in vivo patient-derived xenograft (PDX) model based on zebrafish (Danio rerio) embryos, allowing for a rapid analysis of the angiogenic and invasive potentials, as well as a fast drug sensitivity testing.
Methods Two human gastric cancer cell lines (AGS and SGC-7901) were xenografted into zebrafish embryos, their sensitivity to 5-FU were tested both in vitro and in vivo. Fourteen human primary cells from gastric cancer tissue were xenografted into zebrafish embryos, their proliferating, angiogenic and metastatic activities were evaluated in vivo. Sensitivity to 5-FU, docetaxel, and apatinib were also tested on primary samples from four patients.
Results SGC-7901 showed higher sensitivity to 5-FU than AGS both in vitro (6.3 ± 0.9 μM vs.10.5 ± 1.8 μM) and in vivo. Nine out of fourteen patient samples were successfully transplanted in zebrafish embryos and all showed proliferating, angiogenic and metastatic potentials in the living embryos. Four cases showed varied sensitivity to the selected three chemotherapeutic drugs.
Conclusions Our zebrafish PDX (zPDX) model is a preclinically reliable in vivo model for GC. The zPDX model is also a promising platform for the translational research and personalized treatment on GC.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping