PUBLICATION
Image analysis of neural stem cell division patterns in the zebrafish brain
- Authors
- Lupperger, V., Buggenthin, F., Chapouton, P., Marr, C.
- ID
- ZDB-PUB-171111-1
- Date
- 2017
- Source
- Cytometry. Part A : the journal of the International Society for Analytical Cytology 93(3): 314-322 (Journal)
- Registered Authors
- Chapouton, Prisca
- Keywords
- bioimage informatics, cell identification, neural stem cells, neuroscience image computing, zebrafish brain
- MeSH Terms
-
- Animals
- Cell Division/physiology
- Cell Proliferation/physiology
- Green Fluorescent Proteins/biosynthesis
- Image Processing, Computer-Assisted/methods*
- Neural Stem Cells/cytology*
- Neurogenesis/physiology*
- Telencephalon/cytology*
- Telencephalon/diagnostic imaging*
- Zebrafish
- PubMed
- 29125897 Full text @ Cytometry A
Citation
Lupperger, V., Buggenthin, F., Chapouton, P., Marr, C. (2017) Image analysis of neural stem cell division patterns in the zebrafish brain. Cytometry. Part A : the journal of the International Society for Analytical Cytology. 93(3):314-322.
Abstract
Proliferating stem cells in the adult body are the source of constant regeneration. In the brain, neural stem cells (NSCs) divide to maintain the stem cell population and generate neural progenitor cells that eventually replenish mature neurons and glial cells. How much spatial coordination of NSC division and differentiation is present in a functional brain is an open question. To quantify the patterns of stem cell divisions, one has to (i) identify the pool of NSCs that have the ability to divide, (ii) determine NSCs that divide within a given time window, and (iii) analyze the degree of spatial coordination. Here, we present a bioimage informatics pipeline that automatically identifies GFP expressing NSCs in three-dimensional image stacks of zebrafish brain from whole-mount preparations. We exploit the fact that NSCs in the zebrafish hemispheres are located on a two-dimensional surface and identify between 1,500 and 2,500 NSCs in six brain hemispheres. We then determine the position of dividing NSCs in the hemisphere by EdU incorporation into cells undergoing S-phase and calculate all pairwise NSC distances with three alternative metrics. Finally, we fit a probabilistic model to the observed spatial patterns that accounts for the non-homogeneous distribution of NSCs. We find a weak positive coordination between dividing NSCs irrespective of the metric and conclude that neither strong inhibitory nor strong attractive signals drive NSC divisions in the adult zebrafish brain. © 2017 International Society for Advancement of Cytometry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping