PUBLICATION
Zinc finger protein 365 is a new maternal LPS-binding protein that defends zebrafish embryos against gram-negative bacterial infections
- Authors
- Du, X., Zhou, Y., Song, L., Wang, X., Zhang, S.
- ID
- ZDB-PUB-171019-9
- Date
- 2017
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 32(2): 979-994 (Journal)
- Registered Authors
- Keywords
- antibacterial activity, embryonic immunity, transcriptional factor
- MeSH Terms
-
- Animals
- Bacteria/immunology*
- Bacterial Infections/immunology*
- Embryo, Nonmammalian/immunology*
- Embryo, Nonmammalian/microbiology
- Fish Diseases/immunology*
- Fish Diseases/microbiology
- Transcription Factors/immunology*
- Zebrafish/immunology*
- Zebrafish/microbiology
- Zebrafish Proteins/immunology*
- PubMed
- 29042450 Full text @ FASEB J.
Citation
Du, X., Zhou, Y., Song, L., Wang, X., Zhang, S. (2017) Zinc finger protein 365 is a new maternal LPS-binding protein that defends zebrafish embryos against gram-negative bacterial infections. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 32(2):979-994.
Abstract
Zinc finger protein 365 (ZNF365) is widespread in animals, but its function and mechanism remains poorly defined. Here we clearly demonstrate that zebrafish ZNF365 is a newly identified LPS-binding protein capable of interacting with the gram-negative bacteria Escherichia coli, Vibrio anguillarum, and Aeromonas hydrophila, and functions as an antibacterial effector molecule capable of directly killing the bacteria. We also reveal that N-terminal residues 30-55 consisting of the ZnF_C2H2 domain are indispensable for ZNF365 antimicrobial activity. Importantly, microinjection of recombinant ZNF365 into early embryos significantly enhanced the resistance of the embryos against pathogenic A. hydrophila challenge, whereas down-regulation of ZNF365 by injection of znf365 morpholino into embryos considerably lowered their resistance against A. hydrophila challenge. Furthermore, the N-terminal peptide Z30-55 with in vitro antibacterial activity also promoted the resistance of embryos against A. hydrophila, but the peptide Z56-345 without in vitro antibacterial activity did not. Collectively, these results indicate that ZNF365 is a maternal LPS-binding protein that can protect the early embryos of zebrafish against pathogenic attacks, a novel role to be assigned to ZNF365 proteins. This work also provides new insights into the immunologic function of the zinc finger proteins that are widely distributed in various animals.-Du, X., Zhou, Y., Song, L., Wang, X., Zhang, S. Zinc finger protein 365 is a new maternal LPS-binding protein that defends zebrafish embryos against gram-negative bacterial infections.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping