PUBLICATION

Spatiotemporal image correlation analysis of blood flow in branched vessel networks of zebrafish embryos

Authors
Ceffa, N.G., Cesana, I., Collini, M., D'Alfonso, L., Carra, S., Cotelli, F., Sironi, L., Chirico, G.
ID
ZDB-PUB-171017-11
Date
2017
Source
Journal of Biomedical Optics   22: 1-7 (Journal)
Registered Authors
Cotelli, Franco
Keywords
hemodynamics, image correlation, single plane illumination microscopy
MeSH Terms
  • Animals
  • Blood Vessels/diagnostic imaging
  • Blood Vessels/embryology*
  • Capillaries/diagnostic imaging
  • Capillaries/embryology
  • Computer Simulation
  • Disease Progression
  • Hemodynamics
  • Image Processing, Computer-Assisted
  • Imaging, Three-Dimensional
  • Microcirculation/physiology
  • Microscopy
  • Models, Statistical
  • Morphogenesis
  • Oxygen/chemistry
  • Spatio-Temporal Analysis
  • Spectrophotometry
  • Zebrafish
PubMed
29030941 Full text @ J. Biomed. Opt.
Abstract
Ramification of blood circulation is relevant in a number of physiological and pathological conditions. The oxygen exchange occurs largely in the capillary bed, and the cancer progression is closely linked to the angiogenesis around the tumor mass. Optical microscopy has made impressive improvements in in vivo imaging and dynamic studies based on correlation analysis of time stacks of images. Here, we develop and test advanced methods that allow mapping the flow fields in branched vessel networks at the resolution of 10 to 20  μm. The methods, based on the application of spatiotemporal image correlation spectroscopy and its extension to cross-correlation analysis, are applied here to the case of early stage embryos of zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping