PUBLICATION
Spatiotemporal image correlation analysis of blood flow in branched vessel networks of zebrafish embryos
- Authors
- Ceffa, N.G., Cesana, I., Collini, M., D'Alfonso, L., Carra, S., Cotelli, F., Sironi, L., Chirico, G.
- ID
- ZDB-PUB-171017-11
- Date
- 2017
- Source
- Journal of Biomedical Optics 22: 1-7 (Journal)
- Registered Authors
- Cotelli, Franco
- Keywords
- hemodynamics, image correlation, single plane illumination microscopy
- MeSH Terms
-
- Animals
- Blood Vessels/diagnostic imaging
- Blood Vessels/embryology*
- Capillaries/diagnostic imaging
- Capillaries/embryology
- Computer Simulation
- Disease Progression
- Hemodynamics
- Image Processing, Computer-Assisted
- Imaging, Three-Dimensional
- Microcirculation/physiology
- Microscopy
- Models, Statistical
- Morphogenesis
- Oxygen/chemistry
- Spatio-Temporal Analysis
- Spectrophotometry
- Zebrafish
- PubMed
- 29030941 Full text @ J. Biomed. Opt.
Citation
Ceffa, N.G., Cesana, I., Collini, M., D'Alfonso, L., Carra, S., Cotelli, F., Sironi, L., Chirico, G. (2017) Spatiotemporal image correlation analysis of blood flow in branched vessel networks of zebrafish embryos. Journal of Biomedical Optics. 22:1-7.
Abstract
Ramification of blood circulation is relevant in a number of physiological and pathological conditions. The oxygen exchange occurs largely in the capillary bed, and the cancer progression is closely linked to the angiogenesis around the tumor mass. Optical microscopy has made impressive improvements in in vivo imaging and dynamic studies based on correlation analysis of time stacks of images. Here, we develop and test advanced methods that allow mapping the flow fields in branched vessel networks at the resolution of 10 to 20 μm. The methods, based on the application of spatiotemporal image correlation spectroscopy and its extension to cross-correlation analysis, are applied here to the case of early stage embryos of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping