ZFIN ID: ZDB-PUB-171013-8
Transplantation of Zebrafish Cells by Conventional Pneumatic Microinjector
Shao, M., Cheng, X.N., Liu, Y.Y., Li, J.T., Shi, D.L.
Date: 2017
Source: Zebrafish   15(1): 73-76 (Journal)
Registered Authors: Shao, Ming, Shi, De-Li
Keywords: pneumatic microinjector, transplantation, zebrafish
MeSH Terms:
  • Animals
  • Blastula/cytology
  • Cell Tracking/methods
  • Genes, Reporter*
  • Microinjections/instrumentation*
  • Stem Cell Transplantation/instrumentation*
  • Stem Cell Transplantation/methods*
  • Zebrafish/embryology*
  • Zebrafish/physiology
PubMed: 29023202 Full text @ Zebrafish
Generating chimeric zebrafish by transplantation is extremely useful for live imaging in developmental, stem cell, and cancer biology, and to answer the questions of how cells acquire, keep, and/or change their fate. However, as it is technically challenging, the use of transplantation approach remains very limited by the zebrafish community. In this study, we show that this cell grafting operation can be easily achieved by using a conventional pneumatic microinjector normally used for microinjections. Compared with previously published protocols, which need additional transplantation apparatus, this alternative transplantation method works well, but needs a simpler experimental setup, and is more accessible to all investigators.