PUBLICATION

In Vivo Ribbon Mobility and Turnover of Ribeye at Zebrafish Hair Cell Synapses

Authors
Graydon, C.W., Manor, U., Kindt, K.S.
ID
ZDB-PUB-170809-5
Date
2017
Source
Scientific Reports   7: 7467 (Journal)
Registered Authors
Kindt, Katie
Keywords
Hair cell, Synaptic vesicle exocytosis, Vesicle trafficking
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Movement
  • Fluorescence Recovery After Photobleaching
  • Hair Cells, Auditory/cytology
  • Hair Cells, Auditory/metabolism*
  • Nerve Tissue Proteins/genetics*
  • Nerve Tissue Proteins/metabolism*
  • Synapses/metabolism
  • Synaptic Transmission
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed
28785118 Full text @ Sci. Rep.
Abstract
Ribbons are presynaptic structures that mediate synaptic vesicle release in some sensory cells of the auditory and visual systems. Although composed predominately of the protein Ribeye, very little is known about the structural dynamics of ribbons. Here we describe the in vivo mobility and turnover of Ribeye at hair cell ribbon synapses by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-tagged Ribeye. We show that Ribeye can exchange between halves of a ribbon within ~1 minute in a manner that is consistent with a simple diffusion mechanism. In contrast, exchange of Ribeye between other ribbons via the cell's cytoplasm takes several hours.
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