PUBLICATION

Optoacoustic micro-tomography at 100 volumes per second

Authors
Deán-Ben, X.L., López-Schier, H., Razansky, D.
ID
ZDB-PUB-170802-10
Date
2017
Source
Scientific Reports   7: 6850 (Journal)
Registered Authors
Lopez-Schier, Hernan
Keywords
Microscopy, Optical imaging
MeSH Terms
  • Animals
  • Imaging, Three-Dimensional/methods
  • Imaging, Three-Dimensional/standards
  • Mice
  • Photoacoustic Techniques/methods*
  • Photoacoustic Techniques/standards
  • Tomography, Optical/methods*
  • Tomography, Optical/standards
  • Zebrafish
PubMed
28761048 Full text @ Sci. Rep.
Abstract
Optical microscopy remains a fundamental tool for modern biological discovery owing to its excellent spatial resolution and versatile contrast in visualizing cellular and sub-cellular structures. Yet, the time domain is paramount for the observation of biological dynamics in living systems. Commonly, acquisition of microscopy data involves scanning of a spherically- or cylindrically-focused light beam across the imaged volume, which significantly limits temporal resolution in 3D. Additional complications arise from intense light scattering of biological tissues, further restraining the effective penetration depth and field of view of optical microscopy techniques. To overcome these limitations, we devised a fast optoacoustic micro-tomography (OMT) approach based on simultaneous acquisition of 3D image data with a high-density hemispherical ultrasound array having effective detection bandwidth beyond 25 MHz. We demonstrate fast three-dimensional imaging of freely-swimming zebrafish larvae, achieving 3D imaging speed of 100 volumes per second with isotropic spatial resolution approaching the dimensions of large cells across a field of view exceeding 50mm3. As opposed to other microscopy techniques based on optical contrast, OMT resolves optical absorption acoustically using unfocused light excitation. Thus, no penetration barriers are imposed by light scattering in deep tissues, suggesting it as a powerful approach for multi-scale functional and molecular imaging applications.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping