A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation
- Ma, A.C.H., Shi, X., He, B.L., Guo, Y., Leung, A.Y.H.
- Methods in molecular biology (Clifton, N.J.) 1633: 193-218 (Chapter)
- Registered Authors
- Leung, Anskar
- Hematopoiesis, Mutation, Myeloid malignancy, Myelopoiesis, Transient expression, Zebrafish
- MeSH Terms
- Disease Models, Animal*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- High-Throughput Nucleotide Sequencing
- Isocitrate Dehydrogenase/genetics*
- Leukemia, Myeloid, Acute/genetics*
- Leukemia, Myeloid, Acute/metabolism
- Mutagenesis, Site-Directed
- 28735489 Full text @ Meth. Mol. Biol.
Ma, A.C.H., Shi, X., He, B.L., Guo, Y., Leung, A.Y.H. (2017) A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation. Methods in molecular biology (Clifton, N.J.). 1633:193-218.
The recent advent of next-generation sequencing (NGS) has greatly accelerated identification of gene mutations in myeloid malignancies at unprecedented speed that will soon outpace their functional validation by conventional laboratory techniques and animal models. A high-throughput whole-organism model is useful for the functional validation of new mutations. We recently reported the use of zebrafish to evaluate the hematopoietic function of isocitrate dehydrogenase 1 (IDH1) and the effects of expressing human IDH1-R132H that is frequently identified in human acute myeloid leukemia (AML), in myelopoiesis, with a view to develop zebrafish as a model of AML. Here, we use IDH1 as an example to describe a comprehensive approach to evaluate hematopoietic gene function and the effects of mutations using zebrafish as a model.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes