PUBLICATION
A tunable refractive index matching medium for live imaging cells, tissues and model organisms
- Authors
- Boothe, T., Hilbert, L., Heide, M., Berninger, L., Huttner, W.B., Zaburdaev, V., Vastenhouw, N.L., Myers, E.W., Drechsel, D.N., Rink, J.C.
- ID
- ZDB-PUB-170715-4
- Date
- 2017
- Source
- eLIFE 6: (Journal)
- Registered Authors
- Vastenhouw, Nadine
- Keywords
- cell biology, developmental biology, stem cells, zebrafish
- MeSH Terms
-
- Animals
- Cell Survival
- Embryo, Nonmammalian/physiology
- HeLa Cells
- Humans
- Imaging, Three-Dimensional*
- Models, Biological
- Refractometry/methods*
- Solutions
- Solvents
- Tissue Culture Techniques
- Triiodobenzoic Acids/pharmacology*
- Zebrafish/embryology
- PubMed
- 28708059 Full text @ Elife
Citation
Boothe, T., Hilbert, L., Heide, M., Berninger, L., Huttner, W.B., Zaburdaev, V., Vastenhouw, N.L., Myers, E.W., Drechsel, D.N., Rink, J.C. (2017) A tunable refractive index matching medium for live imaging cells, tissues and model organisms. eLIFE. 6.
Abstract
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus a substantial improvement of the live-imaging of primary cell cultures, planarians, zebrafish and human cerebral organoids.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping