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ZFIN ID: ZDB-PUB-170706-1
Myosin-1 inhibition by PClP affects membrane shape, cortical actin distribution and lipid droplet dynamics in early Zebrafish embryos
Gupta, P., Martin, R., Knölker, H.J., Nihalani, D., Kumar Sinha, D.
Date: 2017
Source: PLoS One 12: e0180301 (Journal)
Registered Authors:
Keywords: Embryos, Zebrafish, Actins, Cell cycle and cell division, Blastomeres, Cell membranes, Membrane staining, Lipids
MeSH Terms:
  • Actin Cytoskeleton/drug effects
  • Actin Cytoskeleton/metabolism
  • Actins/genetics
  • Actins/metabolism*
  • Animals
  • Blastomeres/cytology
  • Blastomeres/metabolism
  • Blastomeres/ultrastructure
  • Blotting, Western
  • Cell Division/drug effects
  • Cell Division/genetics
  • Cell Membrane/metabolism*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/ultrastructure
  • Gene Expression Regulation, Developmental
  • Heterocyclic Compounds, 4 or More Rings/pharmacology
  • Hydrocarbons, Chlorinated/pharmacology
  • Lipid Droplets/metabolism*
  • Microscopy, Electron, Scanning
  • Microscopy, Fluorescence
  • Myosin Heavy Chains/antagonists & inhibitors
  • Myosin Heavy Chains/genetics
  • Myosin Heavy Chains/metabolism*
  • Pyrroles/pharmacology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 28678859 Full text @ PLoS One
Myosin-1 (Myo1) represents a mechanical link between the membrane and actin-cytoskeleton in animal cells. We have studied the effect of Myo1 inhibitor PClP in 1-8 cell Zebrafish embryos. Our results indicate a unique involvement of Myo1 in early development of Zebrafish embryos. Inhibition of Myo1 (by PClP) and Myo2 (by Blebbistatin) lead to arrest in cell division. While Myo1 isoforms appears to be important for both the formation and the maintenance of cleavage furrows, Myo2 is required only for the formation of furrows. We found that the blastodisc of the embryo, which contains a thick actin cortex (~13 μm), is loaded with cortical Myo1. Myo1 appears to be crucial for maintaining the blastodisc morphology and the actin cortex thickness. In addition to cell division and furrow formation, inhibition of Myo1 has a drastic effect on the dynamics and distribution of lipid droplets (LDs) in the blastodisc near the cleavage furrow. All these results above are effects of Myo1 inhibition exclusively; Myo2 inhibition by blebbistatin does not show such phenotypes. Therefore, our results demonstrate a potential role for Myo1 in the maintenance and formation of furrow, blastodisc morphology, cell-division and LD organization within the blastodisc during early embryogenesis.