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ZIRC
ZFIN ID: ZDB-PUB-170602-3
Silica nanoparticles inhibit macrophage activity and angiogenesis via VEGFR2-mediated MAPK signaling pathway in zebrafish embryos
Duan, J., Hu, H., Feng, L., Yang, X., Sun, Z.
Date: 2017
Source: Chemosphere   183: 483-490 (Journal)
Registered Authors:
Keywords: Angiogenesis, MAPK singaling pathway, Macrophage activity, Silica nanoparticles, VEGFR2, Zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified*
  • Cardiovascular Abnormalities/chemically induced
  • Cardiovascular Abnormalities/embryology
  • Embryo, Nonmammalian/blood supply
  • Embryo, Nonmammalian/drug effects*
  • Endothelium, Vascular/drug effects
  • Endothelium, Vascular/embryology
  • MAP Kinase Signaling System/drug effects*
  • Macrophages/drug effects*
  • Macrophages/physiology
  • Nanoparticles/chemistry
  • Nanoparticles/toxicity*
  • Neovascularization, Physiologic/drug effects*
  • Silicon Dioxide/chemistry
  • Silicon Dioxide/toxicity*
  • Vascular Endothelial Growth Factor Receptor-2/genetics
  • Vascular Endothelial Growth Factor Receptor-2/metabolism*
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 28570891 Full text @ Chemosphere
ABSTRACT
The safety evaluation of silica nanoparticles (SiNPs) are getting great attention due to its widely-used in food sciences, chemical industry and biomedicine. However, the adverse effect and underlying mechanisms of SiNPs on cardiovascular system, especially on angiogenesis is still unclear. This study was aimed to illuminate the possible mechanisms of SiNPs on angiogenesis in zebrafish transgenic lines, Tg(fli-1:EGFP) and Albino. SiNPs caused the cardiovascular malformations in a dose-dependent manner via intravenous microinjection. The incidences of cardiovascular malformations were observed as: Pericardial edema > Bradycardia > Blood deficiency. The area of subintestinal vessels (SIVs) was significant reduced in SiNPs-treated groups, accompanied with the weaken expression of vascular endothelial cells in zebrafish embryos. Using neutral red staining, the quantitative number of macrophage was declined; whereas macrophage inhibition rate was elevated in a dose-dependent way. Furthermore, SiNPs significantly decreased the mRNA expression of macrophage activity related gene, macrophage migration inhibitory factor (MIF) and the angiogenesis related gene, vascular endothelial growth factor receptor 2 (VEGFR2). The protein levels of p-Erk1/2 and p-p38 MAPK were markedly decreased in zebrafish exposed to SiNPs. Our results implicate that SiNPs inhibited the macrophage activity and angiogenesis via the downregulation of MAPK singaling pathway.
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