PUBLICATION
Microcystin-LR induces changes in the GABA neurotransmitter system of zebrafish
- Authors
- Yan, W., Li, L., Li, G., Zhao, S.
- ID
- ZDB-PUB-170524-2
- Date
- 2017
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 188: 170-176 (Journal)
- Registered Authors
- Keywords
- GABA, MCLR, Neurotoxicity, Ultrastructure
- MeSH Terms
-
- Animals
- Brain/drug effects
- Brain/metabolism
- Brain/pathology
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- GABA Plasma Membrane Transport Proteins/genetics
- GABA Plasma Membrane Transport Proteins/metabolism
- Glutamate Decarboxylase/genetics
- Glutamate Decarboxylase/metabolism
- Glutamic Acid/metabolism
- Glutaminase/genetics
- Microcystins/toxicity*
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction
- Receptors, GABA-A/genetics
- Receptors, GABA-A/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- gamma-Aminobutyric Acid/metabolism*
- PubMed
- 28535436 Full text @ Aquat. Toxicol.
Citation
Yan, W., Li, L., Li, G., Zhao, S. (2017) Microcystin-LR induces changes in the GABA neurotransmitter system of zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 188:170-176.
Abstract
It has been reported that exposure to microcystins altered adult zebrafish swimming performance parameters, but the possible mechanisms of action remain unknown. Neuronal activity depends on the balance between the number of excitatory and inhibitory processes which are associated with neurotransmitters. In the present study, zebrafish embryos (5 d post-fertilization) were exposed to 0, 0.3, 3 and 30μg/L (microcystin-LR) MCLR for 90day until reaching sexual maturity. To investigate the effects of MCLR on the neurotransmitter system, mRNA levels involved in amino acid g-aminobutyric acid (GABA) and glutamate metabolic pathways were tested using quantitative real-time PCR. Significant increase of GABAA receptor, alpha 1 (gabra1), glutamate decarboxylase (gad1b), glutaminase (glsa) and reduction of mRNA expression of GABA transporter (gat1) at transcriptional level were observed in the brain. Meanwhile, western blotting showed that the protein levels of gabra1, gad1b were induced by MCLR, whereas the expression of gat1 was decreased. In addition, MCLR induced severe damage to cerebrum ultrastructure, showing edematous and collapsed myelinated nerve fibers, distention of endoplasmic reticulum and swelling mitochondria. Our results suggested that MCLR showed neurotoxicity in zebrafish which might attribute to the disorder of GABA neurotransmitter pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping