ZFIN ID: ZDB-PUB-170519-5
Dissection of Larval Zebrafish Gonadal Tissue
Wang, X., Chen, S., Zhang, W., Ren, Y., Zhang, Q., Peng, G.
Date: 2017
Source: Journal of visualized experiments : JoVE (122): (Journal)
Registered Authors: Chen, Sijie, Peng, Gang
Keywords: none
MeSH Terms:
  • Animals
  • Dissection
  • Female
  • Gonads/embryology*
  • Larva/genetics
  • Male
  • Sex Differentiation
  • Transcriptome
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed: 28518099 Full text @ J. Vis. Exp.
Although wild zebrafish possess a ZZ/ZW sex-determination system, domesticated zebrafish have lost the sex chromosome. They utilize a polygenic sex determination system, where several genes distributed throughout the genome collectively determine the sex identities of individual fish. Currently, the genes involved in regulating gonad development and how they work remain elusive. Normally, isolating gonadal tissue is the first step to examine the sex developmental processes. Here, we present a procedure to isolate gonadal tissue from 17 dpf (days post fertilization) and 25 dpf zebrafish larvae. The isolated gonadal tissue may be subsequently examined by morphology and gene expression profiling.