PUBLICATION
Toxic potential of organic constituents of submicron particulate matter (PM1) in an urban road site (Barcelona)
- Authors
- Mesquita, S.R., van Drooge, B.L., Dall'Osto, M., Grimalt, J.O., Barata, C., Vieira, N., Guimarães, L., Piña, B.
- ID
- ZDB-PUB-170517-5
- Date
- 2017
- Source
- Environmental science and pollution research international 24(18): 15406-15415 (Journal)
- Registered Authors
- Piña, Benjamin
- Keywords
- Air pollution, Danio rerio, Gene expression, Organic aerosol, Polycyclic aromatic hydrocarbons, RT-qPCR, Toxicity
- MeSH Terms
-
- Aerosols/toxicity*
- Air Pollutants
- Animals
- Environmental Monitoring
- Gene Expression Regulation/drug effects*
- Humans
- Particle Size
- Particulate Matter/toxicity*
- Risk Assessment
- Spain
- Yeasts
- Zebrafish
- PubMed
- 28508335 Full text @ Environ. Sci. Pollut. Res. Int.
Citation
Mesquita, S.R., van Drooge, B.L., Dall'Osto, M., Grimalt, J.O., Barata, C., Vieira, N., Guimarães, L., Piña, B. (2017) Toxic potential of organic constituents of submicron particulate matter (PM1) in an urban road site (Barcelona). Environmental science and pollution research international. 24(18):15406-15415.
Abstract
Atmospheric particulate matter (PM) is a recognized risk factor contributing to a number of diseases in human populations and wildlife globally. Organic matter is a major component of PM, but its contribution to overall toxicity of PM has not been thoroughly evaluated yet. In the present work, the biological activity of organic extracts from PM1 (particles with less than 1 μm of aerodynamic diameter) collected from an urban road site in the centre of Barcelona (NE Spain) was evaluated using a yeast-based assay (AhR-RYA) and different gene expression markers in zebrafish embryos. Dioxin-like activity of the extracts correlated to primary emissions from local traffic exhausts, reflecting weekday/weekend alternance. Expression levels of cyp1a and of gene markers for key cellular processes and development (ier2, fos) also correlated to vehicle emissions, whereas expression of gene markers related to antioxidant defence and endocrine effects (gstal, hao1, ttr) was strongly reduced in samples with strong contribution from regional air masses with aged secondary organic species or with strong influence of biomass burning emissions. Our data suggest that the toxic potential of PM1 organic chemical constituents strongly depends on the emission sources and on the process of ageing from primary to secondary organic aerosols.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping