PUBLICATION
Benzotriazole ultraviolet stabilizers alter the expression of the thyroid hormone pathway in zebrafish (Danio rerio) embryos
- Authors
- Liang, X., Li, J., Martyniuk, C.J., Wang, J., Mao, Y., Lu, H., Zha, J.
- ID
- ZDB-PUB-170510-1
- Date
- 2017
- Source
- Chemosphere 182: 22-30 (Journal)
- Registered Authors
- Keywords
- Benzotriazole ultraviolet stabilizers, HPT axis-related genes, Thyroid hormone receptor, Toxicological pathways, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Excipients/pharmacology
- Gene Expression Regulation, Developmental
- Metabolic Networks and Pathways/drug effects*
- Receptors, Thyroid Hormone/genetics
- Thyroid Gland/metabolism
- Thyroid Hormones/metabolism*
- Triazoles/analysis
- Triazoles/pharmacology*
- Ultraviolet Rays/adverse effects
- Zebrafish/embryology
- Zebrafish/metabolism*
- PubMed
- 28486152 Full text @ Chemosphere
Citation
Liang, X., Li, J., Martyniuk, C.J., Wang, J., Mao, Y., Lu, H., Zha, J. (2017) Benzotriazole ultraviolet stabilizers alter the expression of the thyroid hormone pathway in zebrafish (Danio rerio) embryos. Chemosphere. 182:22-30.
Abstract
Benzotriazole ultraviolet stabilizers (BUVSs) are widely used in industrial products as well as personal-hygiene products to protect the material or skin from harmful UV-radiation. Due to their persistence and bioaccumulation, BUVSs have been ubiquitously detected in aquatic environments. Although the toxicological effects of BUVSs in aquatic organisms have been previously examined, the effects of BUVSs on the thyroid system have not been adequately addressed. In this study, we assessed putative thyroid disrupting effects of BUVSs (UV-234, UV-326, UV-329 and UV-P) in zebrafish embryos at 1, 10 and 100 μg/L for 96 h. The heart rate was assessed in zebrafish and was observed to be decreased by 6.9%-21.4% in exposure of tested BUVSs. We also observed that the transcript levels of HPT axis-related genes were affected by the 4 BUVSs tested in different ways. Specifically, mRNA levels of thyroid hormone receptors (thraa and thrb) in zebrafish embryos were differentially expressed and the direction of change in these transcripts was isoform and BUVSs dependent. Pathway analysis of the targeted genes measured indicated that cellular processes putatively affected by BUVSs included response to organic substance, regulation of transcription from RNA polymerase II promoter, intracellular receptor signaling pathway, and hypothyroidism. Upon expansion of the network, novel genes involved in this predicted gene network may provide insight into the mechanisms of thyroid disrupting mechanisms of BUVSs. Taken together, our results indicate that BUVSs can potentially impact the thyroid system, and that this is dependent upon the type or structure of BUVSs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping