PUBLICATION

Altered Adipogenesis in Zebrafish Larvae Following High Fat Diet and Chemical Exposure Is Visualised by Stimulated Raman Scattering Microscopy

Authors
den Broeder, M.J., Moester, M.J.B., Kamstra, J.H., Cenijn, P.H., Davidoiu, V., Kamminga, L.M., Ariese, F., de Boer, J.F., Legler, J.
ID
ZDB-PUB-170427-9
Date
2017
Source
International Journal of Molecular Sciences   18(4): (Journal)
Registered Authors
den Broeder, Marjo, Kamminga, Leonie, Legler, Juliette
Keywords
SRS imaging, TBT, TDCiPP, adipocyte, endocrinology, obesity, obesogen, toxicology, zebrafish
MeSH Terms
  • Adipogenesis/drug effects*
  • Animals
  • Cluster Analysis
  • Diet, High-Fat*
  • Environmental Pollutants/toxicity
  • Gene Expression/drug effects
  • Glucose/toxicity
  • Larva/chemistry
  • Larva/drug effects
  • Larva/metabolism
  • Nonlinear Optical Microscopy/methods*
  • Organophosphorus Compounds/toxicity*
  • PPAR alpha/genetics
  • PPAR alpha/metabolism
  • PPAR gamma/genetics
  • PPAR gamma/metabolism
  • Trialkyltin Compounds/toxicity*
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
PubMed
28441764 Full text @ Int. J. Mol. Sci.
Abstract
Early life stage exposure to environmental chemicals may play a role in obesity by altering adipogenesis; however, robust in vivo methods to quantify these effects are lacking. The goal of this study was to analyze the effects of developmental exposure to chemicals on adipogenesis in the zebrafish (Danio rerio). We used label-free Stimulated Raman Scattering (SRS) microscopy for the first time to image zebrafish adipogenesis at 15 days post fertilization (dpf) and compared standard feed conditions (StF) to a high fat diet (HFD) or high glucose diet (HGD). We also exposed zebrafish embryos to a non-toxic concentration of tributyltin (TBT, 1 nM) or Tris(1,3-dichloroisopropyl)phosphate (TDCiPP, 0.5 µM) from 0-6 dpf and reared larvae to 15 dpf under StF. Potential molecular mechanisms of altered adipogenesis were examined by qPCR. Diet-dependent modulation of adipogenesis was observed, with HFD resulting in a threefold increase in larvae with adipocytes, compared to StF and HGD. Developmental exposure to TBT but not TDCiPP significantly increased adipocyte differentiation. The expression of adipogenic genes such as pparda, lxr and lepa was altered in response to HFD or chemicals. This study shows that SRS microscopy can be successfully applied to zebrafish to visualize and quantify adipogenesis, and is a powerful approach for identifying obesogenic chemicals in vivo.
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