PUBLICATION
Inducible Inhibition of Gene Function with Photomorpholinos
- Authors
- Sumanas, S.
- ID
- ZDB-PUB-170402-5
- Date
- 2017
- Source
- Methods in molecular biology (Clifton, N.J.) 1565: 51-57 (Chapter)
- Registered Authors
- Sumanas, Saulius
- Keywords
- Morpholinos Caged, Photoactivatable, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Gene Expression
- Gene Knockdown Techniques
- Gene Silencing*
- Genes, Reporter
- Morpholinos/chemistry
- Morpholinos/genetics*
- Oligonucleotides, Antisense/chemistry
- Oligonucleotides, Antisense/genetics
- Phenotype
- Ultraviolet Rays
- Zebrafish
- Zebrafish Proteins/genetics
- PubMed
- 28364233 Full text @ Meth. Mol. Biol.
Citation
Sumanas, S. (2017) Inducible Inhibition of Gene Function with Photomorpholinos. Methods in molecular biology (Clifton, N.J.). 1565:51-57.
Abstract
Photoactivatable morpholinos (MO) allow specific temporal and spatial inhibition of gene function, which is not possible with conventional morpholino or genetic global gene knock-out approaches. Here, we describe an application of commercially available photoactivatable MO technology for specific gene inhibition in a zebrafish embryonic model and discuss the required controls related to the specificity and efficacy of this method. A similar approach should be also applicable to other model organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping